CD Genomics insists on offering high-quality next generation sequencing (NGS) services to researchers all over the world.
What Is NGS
NGS, also known as high-throughput sequencing, is a DNA sequencing technology which has revolutionized genomic and molecular research and has influenced all fields of biological research, it has become increasingly prevalent in modern society. NGS allows us to sequence DNA and RNA much more accurately, quickly and cheaply.
Library sequencing is a technique that encompasses the initial step of attaching adapters, which include elements like index sequences, barcodes, and sequencing primer binding regions, to both ends of the desired target fragments. Subsequently, these prepared fragments are subjected to sequencing using a dedicated sequencer. In contrast, targeted sequencing is characterized by the selective capture of particular genes or regions of interest before the sequencing phase. Notably, the principal methods for executing targeted sequencing comprise hybrid capture and multiplex PCR.
NGS Services At CD Genomics
With our many years of experience, CD Genomics is a leading provider of NGS services to provide advanced sequencing and bioinformatics solutions for its global customers. Equipped with a wide array of state-of-the-art technologies and supported by a group of experienced professionals, CD Genomics is committed to offering a complete service including experimental design, DNA isolation, DNA fragmentation, library preparation, sequencing, and bioinformatics, as a result, we deliver rapid, cost-effective and high-quality results that enable researchers to achieve scientific breakthroughs.
We are offering both the Illumina sequencing platforms, PacBio Single Molecular Real-Time (SMRT) and Nanopore technologies to meet a broad range of research goals and your budget.
Our technologies and products have been validated through multiple partnerships with the academic institutes, diagnostic companies, and testing laboratories.
Our NGS Solutions Include:
Genome sequencing is the methodology employed to employ high-throughput DNA sequencing techniques alongside advanced bioinformatics tools for the compilation and scrutiny of both the functional and structural constituents of an entire genome. This comprehensive approach facilitates the examination of individual disparities and distinctions within populations. Moreover, by executing de novo sequencing and leveraging bioinformatics methodologies, it becomes possible to derive genetic maps for various species.
The term "transcriptome" encompasses the entirety of transcripts generated by the cells or tissues of a particular species under specific conditions. Leveraging second-generation high-throughput sequencing, it becomes feasible to efficiently and extensively acquire sequence data pertaining to nearly all transcripts and information on differential gene expression within the tissues or organs of a specific species under particular conditions. This approach has found extensive utility across diverse domains, encompassing fundamental research, clinical diagnostics, and pharmaceutical research and development.
Epigenetics, often referred to as epigenomics, epitranscriptomics, or postgenetics, encompasses the investigation of enduring modifications in gene expression or cellular traits that manifest through specific mechanisms, all without modifying the DNA sequence. This realm of inquiry finds application in the realms of biology and specialized genetic disciplines.
Microbiome sequencing encompasses the process of amplifying the notably variable segments of microbial 16S rDNA, 18S rDNA, and ITS, succeeded by high-throughput sequencing. This methodology facilitates the examination of species composition and relative abundance variations among bacteria, archaea, fungi, and other microorganisms present in a given environment. It offers valuable insights into the structural makeup of microbial communities, evolutionary connections, as well as the interrelations between microorganisms and their surroundings within environmental samples.
Metagenomics sequencing, conversely, entails the examination of the genomic constitution of microbial communities within distinct environments, employing high-throughput sequencing techniques. This approach facilitates the exploration of microbial diversity, population dynamics, gene functionality, metabolic networks, and evolutionary connections. Furthermore, it enables the investigation of functional activities within microbial communities, interactions among various microorganisms, and their interplays with the surrounding environment. Research in metagenomics sequencing surmounts the challenges associated with culturing and isolating microorganisms, broadening the scope for the utilization of microbial resources and offering an efficacious instrument for the scrutiny of environmental microbial communities.
Single-cell sequencing technology, in simple terms, is a technique that enables the sequencing and analysis of the genome, transcriptome, and epigenome at the individual cell level. Traditional sequencing is performed at the multicellular level, essentially providing the average signals from a population of cells, thereby missing information related to cell heterogeneity (variations among individual cells). Single-cell sequencing technology, on the other hand, can unveil heterogeneity information that is unattainable with bulk sequencing of mixed samples, effectively addressing this issue.
In addition, we also provide long-read sequencing services
The PacBio Sequel sequencer is Pacific BioSciences' latest third-generation sequencing platform, utilizing Single-Molecule Real-Time (SMRT) sequencing technology. It boasts an average read length of up to 15 kilobases (Kb), with the longest reads extending to 40-60 Kb. The sequencing system comprises 16 SMRT cell sequencing modules, each capable of producing 5-10 gigabases (Gb) of data. Notably, SMRT technology allows for the sequencing of individual DNA molecules without the need for amplification, effectively mitigating PCR-induced GC bias and ensuring comprehensive and uniform sequence coverage. Presently, PacBio SMRT sequencing technology finds widespread applications in complex genome assembly and full-length transcriptome sequencing of eukaryotic organisms.
Nanopore sequencing, as the name suggests, is fundamentally based on the utilization of a nanopore—a tiny pore with covalently attached molecular adapters. The nanopore protein is securely affixed to a membrane with a dynamic protein threading nucleic acids through the nanopore. As a nucleic acid traverses the nanopore, it induces changes in charge, thereby causing fluctuations in the current on the membrane. Due to the exceedingly narrow diameter of nanopores, only individual nucleic acid polymers can pass through. As the electric charge properties of the ATCG individual bases differ, the interference they generate in the current when passing through the protein nanopore varies. By continuously monitoring and decoding these current signals, the determination of the base sequence can be achieved, thus enabling sequencing.
CD Genomics employs the Nanopore platform to conduct thousands of bacterial genome and metagenome sequencing projects, including data analysis. We also perform thousands of full-length transcriptome and Direct RNA sequencing, along with subsequent data analysis.
Advantages of Our NGS Services:
. Highly experienced and qualified personnel
. State-of-the-art instruments and cutting-edge technologies
. Stringent quality control
. Personalized NGS and customized bioinformatics solution to suit your research needs
Professional strategy design
. Broad range of applications
. Competitive price
Surfing to learn how we can help you achieve your research goals through NGS technologies. For more information, please contact us! Our industry-leading Ph.D.-level specialists are always dedicated to providing comprehensive support and technical troubleshooting as quickly as possible.
For Research Use Only. Not for use in diagnostic procedures.
Sample Submission Guidelines
