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Why Conduct Microbiome Sequencing?

Microorganisms are fundamental to ecosystem balance, biodiversity, and a wide range of biological processes that affect agriculture, healthcare, and environmental sustainability. To better understand microbial communities and their functions, researchers focus on three primary levels of microbiome analysis: microbial cultivation, DNA analysis, and mRNA profiling. The development of high-throughput sequencing technologies has revolutionized this field, giving rise to powerful methods such as culture-based genomics (de novo microbial genome sequencing), amplicon sequencing, metagenomics, viromics, and metatranscriptomics. These approaches have rapidly expanded our ability to study complex microbial ecosystems.

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Advanced Microbiome Sequencing Technologies

We use advanced technologies for in-depth microbial genome analysis and comprehensive microbiome studies.

Working with Us

Our end-to-end microbiome sequencing service covers everything from sample preparation to detailed data analysis, ensuring you get the most reliable results to support your research needs.

Report Template for Microbiome Sequencing Services

Download our comprehensive report template to gain insights into your microbial sequencing results, facilitating your research and analysis process. Click the link below to download your template now!

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Customers Often Look For

Lentiviral/Retroviral Integration Site Sequencing

Microbial Diversity Analysis – 16S/18S/ITS Sequencing

Plasmid Identification - Full Length Plasmid Sequencing

SMRT-Based Metagenomics

Viral Metagenomics

Metagenomic Shotgun Sequencing

Microbial Functional Gene qPCR Quantification

Viral RNA Sequencing

PCR-based Microbial Antibiotic Resistance Gene Analysis

Metagenomic Resistance Gene Sequencing Service

Customer Case Publications

Microbiome Sequencing FAQs

• What types of microbial identification services do you offer?

  • CD Genomics offers a comprehensive suite of microbial identification services, encompassing Rep-PCR profiling, Multi-Locus Sequence Typing (MLST), and next-generation sequencing (NGS)-based identification. These advanced methodologies enable precise detection and characterization of microbial strains, thereby facilitating research in diverse fields, including ecological studies, industrial microbiology, and food safety assessments.

• How can microbial functional gene analysis benefit my research?

  • Microbial functional gene analysis is pivotal for elucidating the metabolic profiles of microbial communities. This approach empowers researchers to explore the roles of microorganisms in essential biogeochemical processes such as nutrient cycling, biodegradation, and the emergence of antibiotic resistance. The knowledge derived from these analyses can guide strategies for sustainable environmental management, agricultural productivity, and innovations in biotechnology.

• What is the role of epigenomics in microbial studies?

  • Epigenomics focuses on the chemical modifications of microbial DNA that regulate gene expression without altering the nucleotide sequence. By investigating modifications like 5-methylcytosine (5mC) and N6-methyladenine (6mA), researchers can gain critical insights into microbial adaptability and behavior in response to environmental stimuli, thereby enhancing our understanding of microbial ecology.

• How does your microbial metabolomics analysis work?

  • Our microbial metabolomics analysis emphasizes the comprehensive profiling of metabolites produced by microbial communities. This analytical framework offers valuable insights into the physiological states and interspecies interactions within these communities. The results have broad applicability in fields such as food safety, environmental monitoring, and the exploration of host-microbe dynamics.

• What are the applications of your gut microbiome research solutions?

  • Our gut microbiome research solutions are specifically designed to explore the complex interactions between gut microorganisms and host health. These applications include investigating microbial diversity, examining correlations with metabolic disorders, and evaluating the influence of diet on gut microbiota composition. Ultimately, this research enhances our understanding of the intricate relationships that underlie both human and animal health.

• Why is it important to have biological replicates in the experimental design of a microbial diversity project?

  • In diversity project design, including biological replicates is crucial, particularly for studies focusing on differential analysis. Such analysis often involves both parametric and non-parametric testing methods (e.g., t-tests, rank-sum tests). Both types of tests require calculations such as variance and means, which necessitate biological replicates. Thus, biological replicates are essential from a data analysis perspective. Additionally, environmental samples in diversity research are subject to complex influencing factors, making a single sample per group unreliable. Biological replicates enhance the statistical significance of the research.

• What are the differences between biological replicates, technical replicates, and pooled samples?

  • Biological replicates refer to a set of different samples subjected to the same treatment. They are used to validate generalizable conclusions by summarizing the characteristics of a sample type from the similarity of results among the replicates.

    Technical replicates involve repeating technical operations to account for and verify the stability of experimental results. For instance, sequencing the same soil sample three times would form a set of technical replicates, demonstrating the consistency of the sequencing results.

    Pooled samples are created by mixing multiple samples together, often in equal amounts. The purpose of pooling is to average out inter-sample differences and make the composite sample more representative of the sample type as a whole.

    These three types of sample designs can be combined or used individually, depending on the research objectives. For specific guidance, please consult our technical staff.

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