RNA sequencing (RNA-Seq) is a powerful and high-throughput transcriptome analysis technique utilizing NGS platforms. It can provide accurate estimates of isoform abundance over a large dynamic range. A wide dynamic range allows us to determine both high and low expression levels in individual viral RNA sequencing experiments, greatly increasing the analysis efficiency. Hence, RNA-seq has been very important in basic research. Viral RNA-seq refers to the sequencing of all transcriptional information of viruses, which is time-saving and economical. Furthermore, the use of long-read sequencing (LRS) to solve the complex transcriptome structure has become a popular research method. Here, we have established standardized viral RNA-seq platforms based on NGS, PacBio SMRT, and Oxford nanopore instruments to identify common and novel transcripts without bias. PacBio SMRT sequencing and Oxford nanopore sequencing can generate much longer reads, and detect more transcript overlaps, polycistronic RNAs, splice and transcript variants.
Viral RNA sequencing is important for understanding pathogen-host immune interactions, exploring resistance to specific antibiotics, quantifying changes in gene expression, and monitoring disease progression. Its importance in research has been increasingly recognized. At present, viral RNA-seq using next-generation sequencing and/or long-read sequencing has become a standard method for analyzing viral transcriptome or genome information.
Request a Quote
