We provide next-generation sequencing and long-read sequencing technologies to sequence bacterial whole genome, utilizing the advanced Illumina HiSeq, Nanopore MinION, and PacBio SMRT platforms to produce high-quality sequencing data quickly and efficiently. For next-generation sequencing (NGS), the workflow of bacterial genome de novo sequencing includes four steps: (1) the chromosomal DNA is randomly interrupted; (2) selecting different lengths of sequences to construct a library; (3) large-scale sequencing and genomic assembly; (4) fill in the gap. The final assembly level is measured by the assembly indicators. Bacterial whole genome resequencing is performed based on the high-throughput sequencing data of small fragment libraries and reference-based alignment. For long-read sequencing, long-read libraries can easily cross repetitive and GC-rich segments for accurate detection of chromosome structural variations and fusion genes.
Using bacterial whole genome sequencing, a large number of single nucleotide polymorphisms (SNPs), insertions and deletions (InDels), and structural variations (SVs) can be discovered or identified. Bacterial whole genome sequencing can also be used to identify the unique biological characteristics (pathogenic mechanism, symbiotic mechanism, metabolic mechanism) of the strains and molecular strain typing. Bacterial whole genome sequencing is becoming more relevant to the medical sector, with applications such as diagnostics, epidemiology, risk management, and patient care.
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