Archaeal 16S rRNA Sequencing

Overview

Coupled with bioinformatics analysis, CD Genomics' high-throughput archaeal 16S rRNA gene sequencing can help researchers investigate archaeal diversity, abundance, and phylogeny on a global scale. This service is dedicated to assisting our customers in meeting both scientific and regulatory requirements.

Our Advantages:

High accuracy: Truly reflecting the evolutionary relevance of organisms and groups of organisms.
Flexibility: The variable regions (such as V1-V3, V3-V4, V4-V5, V4) of 16S rDNA or the full-length 16S rRNA are sequenced and analyzed.
A range of solutions, including microbial identification and abundance, community structure analysis, phylogenetic analysis, etc.
Multiple applications, including industrial manufacturing, drug discovery and development, agriculture, environmental protection, etc.
More efficient: Compared with traditional research methods, sequencing can generate larger output data in a high throughput manner.

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We are dedicated to providing outstanding customer service and being reachable at all times.

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Introduction to our Archaeal 16S rRNA sequencing

The 16S rRNA gene is a highly conserved component of all DNA-based life forms and thus is highly suited as a molecular marker for sequencing DNA in samples containing thousands of different species. Because of the difficulty of cultivating archaeal compared with bacteria, archaeal 16S rRNA sequencing is a preferred method to study the origin and evolutionary relationship of archaeal. To better serve your need, we have advanced our next-generation sequencing (NGS) platforms to include instruments such as Illumina Hiseq2500, HiSeq 4000, Miseq, and Roche 454 GS FLX platform, as well as third-generation sequencing platforms such as PacBio RS II and Nanopore instruments. Based on our diverse sequencing platforms and expertise in microbial genomics, we are dedicated to providing (full-length) 16S rRNA gene sequencing for identification, classification, and quantitation of archaeal within complex biological mixtures such as environmental samples.

We provide comprehensive bioinformatic analyses including original sequence quality control, OUT clustering, taxonomic classification, diversity analysis, abundance analysis, and phylogenetic analysis. Functional analysis can also be carried out for the biological significance of archaea, such as their beneficial or potentially pathogenic effects in the human microbiome. We provide guidance along your way to discover new archaea and understand their biological functions. At present, there are only less than 1000 archaea genomic data in the National Center of Biotechnology Information (NCBI) database, which is slightly insufficient compared with the actual biodiversity. Since archaea are found in soil human bodies, and extreme environment, archaea has important implications for human, animal, and plant health.

Archaeal 16S rRNA sequencing workflow

Bioinformatics Analysis

Our bioinformatics analysis includes five parts: species annotation, diversity analysis, evolutionary analysis, functional profiling, and correlation analysis. We are flexible to your needs.

Bioinformatics Analysis		The Problem to be Solved
Quality control		Read quality filtering, quality trimming, OTU filtering
Species annotation		OTU clustering, species annotation
Diversity analysis	α diversity	Diversity index; rarefaction curve
	β diversity	Sample differences
	Meta-analysis	Comparative studies of abundant features between datasets
Evolutionary analysis		Construction of phylogenetic trees
Functional analysis		Prediction of the biological significance of archaea
Custom analysis		Custom analysis based on customer needs

Sample Requirement

1. DNA amount ≥ 100 ng, PCR products ≥ 400 ng, concentration ≥ 10 ng/μL, 1.8 < OD_260/280 < 2.0

Sampling kits: We provide a complete range of microbial sampling kits for clients, including microbial collection products, DNA/RNA isolation kits, and accessories for storage and mailing.

Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report.

References

Fillol Homs M. Insights into the distribution and ecological role of members of the archaeal Phylum Bathyarchaeota. From the global to the local scale. 2017.
Koonin E V, Yutin N. The dispersed archaeal eukaryome and the complex archaeal ancestor of eukaryotes. Cold Spring Harbor perspectives in biology, 2014, 6(4): a016188.
Nicks T, Rahn-Lee L. Inside out: Archaeal ectosymbionts suggest a second model of reduced-genome evolution. Frontiers in Microbiology, 2017, 8: 384.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.