Accelerate Research and Practice in Petroleum microbiology

Whole Genome Sequencing (WGS) provides actual DNA sequence data for an organism's whole genome It is focused on next-generation sequencing (NGS), also identified as high sequencing, which is an innovation for vastly parallel DNA sequencing that enables specimens and processes to be analyzed at a stage that has never been feasible before. NGS systems can more cost-effectively sequence huge specimens than conventional ways, and produce more comprehensive genomic information in the procedure.

DNA biomarkers such as single nucleotide polymorphism (SNPs), insertions and deletions (indels), structure variations (SVs), copy number variations (CNVs), and other high precision genetic modifications of the sequenced organisms can be identified by whole-genome resequencing. It also offers an unparalleled chance to characterize a community's polymorphic versions, which concisely untangle the underlying frameworks of origin, advancement, development, and transformation of species.

It is also possible to evaluate complicated bacterial/fungal mixed communities through WGS. Using so-called barcoding areas in the microbial DNA, our microbial screening services allow an overview into the microflora of petroleum specimens thus classifying the genus or species. Our metagenomics service looks at the total DNA of a complicated specimen involving combined communities, enabling strains and subspecies to be identified. Even non-cultivable microorganisms are included and identified in both methodologies.

Services

• Detection at a level of single base-pair resolution
• Sequencing de novo and classification of genome-wide mutations
• Evolution and phylogenetic experiments of populations
• Study on the development of industrial applications

Sample Requirements

• 

  1. The samples that will be submitted must satisfy the following criteria: DNA amount ≥ 2 μg, DNA concentration ≥ 20 ng/μl, 1.8 < OD_260/280 < 2.0, no degradation or contamination. For purity and volume, all DNA specimens are verified.
  2. Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), and your designated bioinformatics report, including taxonomic assignment, functional annotation, microbial community structure analysis, prediction of bacteriophage sequences, statistics analysis, etc.