Fungal Whole Genome De Novo Sequencing

Overview

Fungi are crucial to the biosphere and human health due to their important medical and economic value. Some fungi are closely related to animals, plants, and other life forms by developing symbiotic relationships. Fungi also produce some secondary metabolites like penicillin, cyclosporine, and lovastatin, which have vital medicinal, agricultural, or cosmetic prosperities. Fungal whole genome de novo sequencing can help us better understand the fungal biodiversity, physiology, metabolism, and function. Our fungal whole-genome de novo sequencing based on next-generation sequencing (NGS) and long-read sequencing technologies can assist you to explore novel fungal species in an effective way.

Our Advantages:

The least degree of bias and longer read lengths (average > 15,000 bp, some reads >100,000 bp) generated by long-read sequencing instruments.
Advanced sequencing platforms: Illumina HiSeq, Nanopore MinION, and PacBio Sequel platforms.
Extensive experience in the industry of genomic service.

Tell Us About Your Project

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Introduction to our fungal whole genome de novo sequencing platform

We utilize both long-read and short-read sequencing technologies for fungal whole genome de novo sequencing. Both PacBio and Nanopore platforms are superior long-read sequencing platforms that generate reads with tens of kilobases in length for better genome reconstruction. A single molecule of DNA can be sequenced without the need for PCR amplification or chemical labeling of the sample. De novo assembly from long reads can span the low complexity and repetitive regions and create accurate assemblies. Additionally, we perform fungal whole genome de novo sequencing on Illumina HiSeq, which is a short-read sequencing platform with high accuracy.

Fungal whole genome de novo sequencing, which is used to explore novel fungal populations, enables the fungal genomes reconstruction, functional analysis, and evolutionary studies. This technique can provide insight into the biological diversity of fungi and yeast, thereby expanding research into agricultural science, ecology, bioremediation, medical science, cosmetic science, bioenergy, and biotechnology industry. We also provide fungal whole genome resequencing to detect variants, explore gene functions, and reconstruct phylogenetic trees.

Project Workflow

Bioinformatics Analysis

Our bioinformatics analysis includes three parts: genome annotation, comparative genomic analysis and gene function analysis, which are flexible to your needs.

Genome Annotation	Repetitive sequence analysis
	Detection of CRISPRs
	Non-coding RNA prediction
	Genomic islands prediction
	Prophage prediction
	Gene structure analysis
Comparative Genomic	Synteny analysis
	Common and specific genes
	Evolutionary biology
	Gene family anlaysis
Gene Function Analysis	Functional annotation
	Analysis of pathogenicity and drug resistance
	Prediction of secreted protein

Sample Requirement

1. Illumina platform: DNA/cDNA amount ≥ 2 μg, concentration ≥ 20 ng/μL
2. PacBio platform: DNA amount ≥ 10 μg, concentration ≥ 80 ng/μL
3. DNA purity: OD_260/280 =1.8 ~2.0; No degradation or RNA contamination

Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.

Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.