Inquiry
Request a Quote

Eukaryotic Diversity Analysis

Overview

On the basis of Operational Taxonomic Unit (OTU), our eukaryotic diversity analysis platform is dedicated to characterizing eukaryotic diversity in an efficient and economical manner. We utilize the advanced next/third generation sequencing technology in combination with molecular biology methods to support our clients in a variety of fields.

Our Advantages:
  • Extensive experience.
  • Targeted region sequencing, enabling a rapid and efficient workflow.
  • More than 70% of our team is PhD and Masters-level scientists.
  • Both Illumina PE250/300 and PacBio systems are available.
Tell Us About Your Project

We are dedicated to providing outstanding customer service and being reachable at all times.

Request a Quote

Introduction to Our Eukaryotic Diversity Analysis

Our integrated and novel platform enables high-throughput and reliable 18S/ITS analysis for eukaryotic diversity detection with a combination of sequencing technology (Sanger, Illumina MiSeq/HiSeq, and PacBio SMRT sequencing) and quantitative PCR (q-PCR). The principle of this platform is based on the role of 18S rRNA and ITS as molecular markers in eukaryotes. This platform allows both short-read 18S/ITS sequencing by Illumina MiSeq/HiSeq (such as the amplification and sequencing of V4 region of 18S rRNA) and full-length 18S/ITS sequencing by PacBio SMRT technology. The data need to be further validated by Sanger sequencing and q-PCR with genus- and species-specific primers. Quantitative PCR can also be used to quantify microbial strains. Based on our eukaryotic diversity analysis platform, we provide provide eukaryotic 18S rRNA sequencing and fungal ITS sequencing.

Our platform has supported a variety of fields, including basic research, agriculture, environment, as well as pharmaceutical and food & beverage industries, etc. Our featured eukaryotic diversity analysis services include soil actinomycetes diversity analysis and clinical microbiology. In addition to eukaryotic diversity analysis platform, prokaryotic diversity analysis platform is also available for comprehensive microbial diversity detection by using 16S rRNA as molecular marker.

Eukaryotic diversity analysis workflow

Bioinformatics Analysis

Our experts conduct reliable eukaryotic diversity analysis including species annotation, diversity analysis, evolutionary analysis, functional profiling, and correlation analysis. The following table shows the optional bioinformatics analyses at CD Genomics.

Table 1. Eukaryotic diversity analysis at CD Genomics.

Bioinformatics analysis Problems to be solved
Species annotation OTU clustering, species annotation
Diversity analysis α diversity Species richness within a single microbial ecosystem
β diversity Diversity in microbial community between different environments
Meta-analysis Comparative studies of abundant features between datasets
Evolutionary analysis Construction of phylogenetic tree
Functional analysis Analysis of the Intestinal Microflora Locate the gut bacteria, predict human disease, and provide disease treatment
PICRUSt Predict functional profiles
FAPROTAX Predict the process of biochemical cycles
Tax4fun Predict functional profiles
FunGuild Predict the ecological roles of eukaryotes
Correlation analysis CCA Study the relationships between microbes and environmental factors
VPA
Network analysis

Sample Requirement

  • OD260/230 ≥ 1.8; 2.0, 1.8 < OD260/280 < 2.0, no degradation or contamination.
    Illumina platform: DNA amount ≥ 300 ng, PCR Products ≥ 400 ng
    PacBio platform: gDNA ≥ 100 ng, PCR Products ≥ 400 ng

Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.



microbioseq

Follow us on:

Services
Solutions  Inquiry
Copyright © 2024 CD Genomics. All rights reserved. Terms of Use | Privacy Notice