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Antibiotic Resistance Genes (ARGs) Analysis Solution
TaqMan-based qPCR Assay for ARGs

TaqMan-based qPCR Assay for ARGs

Overview

The widespread use of third- and fourth-generation cephalosporins, carbapenems, and fluoroquinolones, among other ultra-broad-spectrum antibiotics, has led to an epidemic of antibiotic-resistant bacteria in the environment. Antibiotic resistance genes are an emerging environmental contaminant that can enter pathogenic bacteria and confer host resistance to antibiotics, including through horizontal gene transfer, thereby posing a serious threat to human health. To enable rapid and accurate detection of drug-resistant bacteria and control of hospital-acquired infections, CD Genomics offers comprehensive antibiotic resistance gene (ARGs) detection solutions to detect drug resistance genes. TaqMan-based real-time PCR technology is certainly a good technical tool to address this problem.

Our Advantages of TaqMan:

It is unaffected by non-specific amplification and primer dimerization.
It has the advantages of easy operation, high sensitivity, good specificity, and high accuracy.
It allows timely monitoring and analysis of microbial antibiotic resistance and interruption of nosocomial infections.
Using different probes, multiple ARGs can be detected simultaneously in one system using different fluorescent markers.

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Introduction to Our TaqMan-based qPCR Analysis Platform

TaqMan-based qPCR Assay for ARGs

TaqMan-based qPCR is often used to detect antibiotic resistance genes in microorganisms, and it is a recognized efficient and accurate method. TaqMan-based qPCR needs to add a specific fluorescent probe while adding a pair of amplification primers. TaqMan probes are linear oligonucleotides labeled with a fluorescent reporter group (FAM or Hex, etc.) and a fluorescent quencher group at both ends. When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quencher group, and the PCR instrument cannot detect the fluorescent signal. During PCR amplification (during the extension phase), the 5'-3' exonuclease activity of Taq enzyme degrades the probe and separates the reporter fluorophore from the quencher fluorophore, so that the fluorescence monitoring system can receive a Fluorescent signal, that is, one fluorescent molecule is formed for each DNA strand amplified. The accumulation of fluorescent signals is fully synchronized with the formation of PCR products.

Workflow of TaqMan-based qPCR Analysis of ARGs

The Flowchart of TaqMan-based qPCR Assay for ARGs - CD Genomics.

What We Offer

CD Genomics is committed to the analysis of antibiotic resistance genes in microorganisms. With rich experience, it can provide you with TaqMan-based qPCR solutions for hundreds of antibiotic resistance genes. The following table only shows some of the target genes that we can compete. For more information, please contact us directly.

Gene	Classification	Mechanism
GES, KPC, IMP-1, NDM-1(C), blaOXA-48, etc	Carbapenems	-
vanA, vanB, vanC, vanG, vanHB, vanHD, vanRA, vanRB, vanRC, vanRD, vanSA, vanSB, vanSC, vanTC, vanTE, vanTG, vanWB, vanWG, vanXA, vanXB, vanXD, vanYB, vanYD, etc	Vancomycin	protection
acrA, adeA, acrF, ceoA, cmeA, cmr, marR, mdetl1, mdtE/yhiU, mepA, mexA, mexD, mexE, mexF, mtrC, mtrD, oprD, oprJ, pmrA, qac, qacA, qacA/qacB, qacH, rarD, sdeB, tolC, ttgB, yceE/mdtG, yceL/mdtH, yidY/mdtL, ttgA, emrD, etc	Multidrug	efflux

Bioinformatics Analysis

Depending on your needs, we will develop a tailor-made bioinformatics analysis solution for you.

ANALYSIS CONTENTS	DETAILS
Analysis of antibiotic resistance in bacteria	Analysis of carbapenem resistance in Escherichia coli, Klebsiella pneumoniae, and A. baumannii.
Quantitative analysis	Quantification of the abundance of target antibiotic resistance genes.
Absolute quantification	Absolute quantification of antibiotic resistance gene abundance.
Gene expression analysis	Analysis of resistance gene expression for carbapenems, β-lactams, quinolones, and other antibiotics.

Sample Requirements

1. DNA: 1.8 < OD260/280 < 2.0, no contamination or degradation. Please guarantee that specimens are properly secured and packaged during transport.

Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.

References

Manchanda V, Rai S, Gupta S, et al. Development of TaqMan real-time polymerase chain reaction for the detection of the newly emerging form of carbapenem resistance gene in clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii. Indian J Med Microbiol. 2011 Jul-Sep; 29(3): 249-53.
Li Y, Shen Z, Ding S, et al. TaqMan-based multiplex real-time PCR assay for the rapid detection of tigecycline resistance genes from bacteria, faeces and environmental samples. BMC Microbiol. 2020 Jun 22; 20(1): 174.