CD Genomics offers specialized Microbial Short-Chain Fatty Acid (SCFA) Analysis with high specificity and accuracy. Utilizing advanced GC-MS technology, we provide precise identification and quantification of SCFAs, delivering rapid results through optimized chromatographic methods. This analysis is essential for understanding gut health, metabolic disorders, and the role of microbiota in various diseases.
Our Advantages:
- High Specificity and Accuracy: GC-SIM for precise identification and absolute quantification.
- Custom Kit: Specifically developed for short-chain fatty acids.
- Rigorous Quality Control: Internal and external standards, manual calibration of standards, calibration curve R² > 0.9.
- Standardized Methods: Integration of advanced metabolomics and genomic analyses.
- Rapid Separation: Use of longer chromatographic columns and higher carrier gas speeds.
- High Reliability: Consistent performance with continuous operation capability.
Introduction to Short-Chain Fatty Acids (SCFAs)
SCFAs are critical metabolites produced through the fermentation of dietary fibers and resistant starches by the gut microbiota. The primary SCFAs include acetate, propionate, and butyrate. These fatty acids play indispensable roles in various physiological processes: they supply energy to colonocytes, fortify gut barrier integrity, modulate immune functionality, and influence systemic metabolism. The presence and activity of SCFAs contribute significantly to maintaining a healthy gut environment and are intricately involved in metabolic regulations. Moreover, SCFAs are associated with several health conditions, including inflammatory bowel disease and metabolic syndrome, underscoring their importance in preserving overall health and preventing disease.
What is SCFA Analysis
The analysis of SCFAs entails quantifying their concentrations and types within biological samples such as feces, blood, or tissues. This type of analysis is fundamental for providing insights into an individual's metabolic health and the condition of their gut microbiota.
Techniques for SCFA Analysis:
- Gas Chromatography-Mass Spectrometry (GC-MS): This widely-used technique excels in detecting and quantifying SCFAs, offering both high sensitivity and specificity. By employing Selected Ion Monitoring (SIM), GC-MS enhances its capability to detect even low concentrations of SCFAs within complex sample matrices.
- High-Performance Liquid Chromatography (HPLC): When paired with UV detection (HPLC-UVD) or alternative detectors, HPLC serves as an effective method for analyzing SCFAs. This technique provides excellent resolution and manages complex samples efficiently.
- Capillary Electrophoresis (CE): Known for its rapid analysis and minimal sample preparation requirements, CE is suitable for routine measurements of SCFAs and offers a swift and efficient option for SCFA quantification.
These methodologies collectively contribute to the comprehensive understanding of SCFA profiles, reflecting the metabolic status and gut microbiota health of individuals.
Applications of SCFA Analysis
The applications of SCFA analysis include, but are not limited to, the following areas:
- Animal disease research
- Food science, nutrition, and developmental research
- Early disease biomarker discovery
- Gut microbiota research
- Disease research
- Mechanistic research on metabolic regulation
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Figure 1 Comparison of Fecal Levels of Short Chain Fatty Acids in Patients With PD and Healthy Controls.
Figure 2 ROC Curves for Distinguishing Patients With PD From Controls and Correlations Between SCFA Levels and Clinical Motor Symptom Severity in Patients With PD.
