Microbial gyrB Genotyping Service

Overview

The gyrB gene encrypts the DNA gyrase subunit B protein, a type II DNA topoisomerase that performs an important part in the replication of DNA and is widely dispersed amongst bacterial organisms. The percentage of molecular evolution implied from sequences of the gyrB gene is faster than that implied from sequences of the 16S rRNA gene. In phylogenetic research of Pseudomonas, Acinetobacter, Mycobacterium, Salmonella, Shigella and Escherichia coli, Aeromonas and the Bacillus anthracis-cereus-thuringiensis group, gyrB gene sequences have been used findings from the research have shown that gyrB is an appropriate phylogenetic marker for the research at the species level of phylogenetic and taxonomic interactions. It has been indicated in the current analysis that direct gyrB gene sequencing could be utilized for the classification and phylogenetic assessment.

Our Advantages

No previous genome sequence information necessary
Price-efficient
Skillful service and fast turnaround instances
Suitable with high-throughput KASP genotyping
Sustain bioinformatics for any organisms
Other characteristics involve decreased sample management, less PCR & purification process, no fractionation of DNA size, effective barcoding scheme, exploration of simultaneous markers & genotyping

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We are dedicated to providing outstanding customer service and being reachable at all times.

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Introduction to Our Microbial gyrB Genotyping Platform

Microbial gyrB Genotyping Service

Over the decade, CD Genomics has provided precise and inexpensive genotyping through sequencing offerings. The newest Illumina sequencing systems are used to acquire data on genetic variants in a more effective method. Genotyping by Sequencing (GBS), also defined as Sequence-Based Genotyping (SBG), has become the preferred technique for multiple DNA specimens for greater discovery of SNPs and concurrent genotyping. It integrates the lowering of enzyme-mediated complexity with the high-throughput sequencing capability of Illumina frameworks to determine random markers across an entire genome.

Microbial gyrB Genotyping Service Workflow

Microbial gyrB Genotyping Service

Bioinformatics Analysis

We provide sustain bioinformatics for any organisms: such as pre-processing of sequences, coordination, and variant assessment.

ANALYSIS CONTENT	DETAILS
GATK	Identifying SNPs and indels in DNA and RNAseq germline info
TASSEL-GBS	The identification and calling of next-generation SNPs
Analysis of NGS reads	Graded genotyping data supplied on the proposal as conventional, raw sequence info (FASTQ template)
Burrows-Wheeler Aligner (BWA)	Plotting of low-divergent sequences against a big genome of reference
Bowtie 2	Sequencing alignment reads for long reference sequences
Probe design and probe library generation	Assay model utilizing customer-provided data on SNP sequences

Sample Requirements

1. 1.5 μl of DNA per sample per SNP at a suitable composition for your research organism's genome size. This volume is computed based on the least composition the volume necessity is lowered if higher concentrations are provided. We do not accept volumes of less than 20 μl because of laboratory handling protocols. You will still need to provide 20 μl of starting items if you only have a tiny proportion of SNPs to operate.

Sampling kits: We provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.

References

Wickland DP, Battu G, Hudson KA, et al. A comparison of genotyping-by-sequencing analysis methods on low-coverage crop datasets shows advantages of a new workflow, GB-eaSy. BMC bioinformatics. 2017, 18(1):586.
Perea C, De La Hoz JF, et al. Bioinformatic analysis of genotype by sequencing (GBS) data with NGSEP. BMC genomics. 2016, 17(5).
Elshire RJ, Glaubitz JC, Sun Q, et al. A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS One. 2011.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.