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Microbial Functional Diversity Analysis

Overview

Microbial species can be characterized into functional groups that exhibit similar responses to the environment or show similar effects on major ecosystem processes. Functional diversity refers to the range and value of biological traits that affect ecosystem properties. This can be expressed in many ways, including the number and relative abundance of functional groups, the diversity of interactions with ecological processes, or the average differences in functional-related characteristics among species.

Our Advantages:
  • Integration of many specimens in a single sequencing run and achieve functional diversity analysis.
  • Professional, comprehensive, and customized analysis pipeline.
  • Standardized laboratory methods and strict quality control.
  • High-resolution sequencing coupled with optimized bioinformatics analysis.
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Introduction to Our Microbial Functional Diversity Analysis Services

Research indicates that microbes are essential and play a vital role in the ecosystem in these environments. It draws attention to the analysis of a complicated microbial community's taxonomic structure. Shotgun sequencing has been established to use random fragments as a fast-sequencing method. In all organisms within a bacterial diversity, metagenomic shotgun sequencing is a fast and effective method for gaining all genetic data. The whole microbial genetic information enclosed in an environmental specimen is targeted by metagenomic shotgun sequencing. It is possible to further correlate the acquired community taxonomic profile with the functional profile of known and unknown lines of organisms. In sequenced genomes, the full sequences of protein-coding genes and complete operons may provide invaluable functional information of the microbial communities living in realistic ecosystems under research. Metagenomic shotgun sequencing can give genetic information on potentially novel biocatalysts or enzymes, uncultured organisms' genomic links between mechanism and phylogeny, evolutionary community feature and structure profiles, and much more.

Shotgun metagenomic sequencing enables all genes in all organisms present in a specified complicated specimen to be concisely sampled by researchers. The technique allows microbiologists in different environments to assess microbial communities and identify the abundance of microbes. It also offers a means of studying unculturable microorganisms that are hard or impossible to evaluate otherwise. CD Genomics is dedicated to delivering qualified metagenomic shotgun sequencing services as an experienced supplier of NGS services. Shotgun metagenomic sequencing enables cheap and time-efficient taxonomic and biological functional classification of polymicrobial populations. With flexibility and bioinformatics analysis based on customers' requirements, we continuously produce high-quality data.

Microbial Functional Diversity Analysis Workflow

Bioinformatic Analyses

Analysis Contents Details
DNA Assembly For both cloning and synthetic biology (in which genes and proteins are remodeled and/or constructed into new methods of generating new and beneficial functionality), DNA assembly can be used.
Functional Metagenomics New functional genes, microbial routes, antibiotic resistance genes, and functional abnormalities of microbial groups can be recognized by functional metagenomics. And interaction and co-evolution between microbiota and hosts also be determined.
Heatmap Offers visualization of data for multiple tasks, such as microarray and RNA-Seq data differential expression evaluations.
Species Annotation Classifying functional components along a genome's sequence. It enables DNA sequencing, which then generates sequences of unknown functions.
Alpha and Beta Diversity We quantify alpha-diversity within a habitat sort as the observed richness (number of taxa) or evenness (relative abundances of those taxa) of an average sample. We also measure beta-diversity among specimens within a habitat as the variance in the structure of the population (the identity of taxa observed).

Sample requirement

    1. Specimens from human, animal, natural and industrial environments, as well as DNA and PCR products.
    2. 1.8 < OD260/280 < 2.0, OD260/230 ≥ 1.8, no degradation or contamination.
    3. Prokaryotic/eukaryotic DNA/cDNA amount ≥ 2 μg, viral DNA/cDNA amount ≥ 1 ug

Deliverables: Raw sequencing data (FASTQ), trimmed and stitched sequences, quality-control dashboard, statistic data, and your designated bioinformatics report.

* For Research Use Only. Not for use in diagnostic procedures or other clinical purposes.



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