Microorganisms are widely used in large-scale industrial processes to produce a variety of metabolites, like lactic acid and ethanol. Many laboratories and bio-industries need to characterize microbial strains to make better use of microorganisms. Microorganisms can contain multiple copies of a gene, cassette or operon, which can be found especially in fungi. The number of gene copy may affect the expression levels of the corresponding proteins and provides valuable insights into production strain optimization. Transgenic models are very useful, but transgenes typically insert randomly in the genome and the number of copies varies, leading to copy number variations (CNVs). Therefore, it is very important to ensure the consistent phenotype of each colony. CD Genomics developed a robust and sequencing-based platform to help scientists and biotechnology companies quickly and comprehensively characterize microbial strains.
NGS and long-read sequencing technologies offer a strong potential for microbial copy number analysis. Based on Illumina HiSeq/MiSeq, PacBio or Oxford nanopore sequencing platforms, we are able to determine the copy number of each transgene, thus providing quality control of your transgenic models. Long-read sequencing technologies is a powerful tool to cover complex repetitive regions. Further data analysis helps explain the relationship between transgene copy number and changes of phenotype or inconsistent performance of microbial strains. These information are crucial for understanding disease phenotype and drug efficacy analysis.
Request a Quote
