NextSeq 500 Sequencer

Highlights of NextSeq 500 Sequencer:

• Unprecedented Speed and Throughput: Generate 1.6-1.8 Tb in < 3 days in dual flow cell mode to perform whole-genome sequencing on an unrivaled scale.
• Exceptional Data Quality: Highly accurate Illumina sequencing by synthesis (SBS) chemistry delivers proven industry-leading data quality.
• Get accurate bi-directional amplicon sequencing
• Population-Scale Genome Sequencing: Highest daily throughput delivers unmatched cost-effectiveness for population-scale projects.
• Throughput Range: The NextSeq 500 allows adjustable throughput to meet diverse needs, offering both High Output and Mid Output modes. In High Output mode, it can generate up to 120 Gb of data per run, suitable for large-scale projects, while the Mid Output mode can generate up to 39 Gb per run, ideal for smaller and targeted sequencing projects.
• Variable Read Lengths: Supports multiple read length configurations such as single-end 75 bp, paired-end 75 bp, single-end 150 bp, and paired-end 150 bp, providing versatility for different experimental requirements.
• One-Step Workflow: From sample preparation to data analysis, the NextSeq 500 features a streamlined workflow. Automated sample loading and a user-friendly interface make operation straightforward, even for beginners.
• Quick Turnaround: With a short run time of just 11-29 hours (depending on the chosen throughput and read length), the NextSeq 500 significantly enhances lab efficiency.
• High Accuracy: Utilizing Illumina's SBS technology, the NextSeq 500 delivers highly accurate sequencing. Dual molecular indexing further reduces sequencing errors, ensuring data reliability.
• Deep Coverage: Suitable for applications requiring high coverage, such as variant detection, copy number variation analysis, and rare mutation identification.

NextSeq 500 Sequencer Performance Parameters

Applications of NextSeq 500 Sequencer:

The NextSeq 500 is suitable for a wide range of applications, including but not limited to:

• Genomics: Small genome sequencing, large-scale exome sequencing, genome-wide association studies (GWAS).
• Transcriptomics: RNA-seq, single-cell RNA-seq, quantitative transcriptome analysis.
• Epigenomics: ChIP-seq, ATAC-seq, methylation sequencing.
• NextSeq 500 Sequencer facilitates high-throughput genotyping by sequencing, enabling the simultaneous analysis of thousands to millions of genetic variants across genomes.
• Clinical Research: Cancer research, genetic disease diagnosis, pharmacogenomics.
• Genotyping by Sequencing
• Metagenomics

Comparison of NextSeq 500 Sequencer and Illumina NovaSeq 6000:

The NextSeq 500 Sequencer stands as a versatile and efficient tool for mid-sized laboratories, offering high-throughput capabilities, operational simplicity, and superior data quality. Suitable for applications spanning basic research to clinical studies, the NextSeq 500 is a dependable platform that equips researchers to delve into the intricate realms of scientific inquiry.