Microorganisms such as bacteria, virus, and fungi am teeming in the air. These biological particles contributes to the ecological balance and play an important role in agriculture, the biosphere, cloud formation, global climate, and atmospheric dynamics. However, they also affect the environmental equality, and even act as a medium for the spread that leads to air pollution and human diseases. Airborne microorganisms are a major cause of human respiratory diseases, such as asthma, allergies, and pathogenic infections. Both cultivation-dependent and –independent methods can be used to explore microbial diversity in the air, uncovering the potential impacts on the natural environment and human disease.
Request a QuoteWe analyze the microbiome of various environments including indoor and outdoor air, and their impact on human health using sequencing techniques like metagenomics sequencing, 16S/18S/ITS sequencing, and metranscriptomic sequencing. RT-qPCR is also used for achieving the qualitative and quantitative analysis of DNA or RNA. Bioinformatics analysis is required to process raw reads and determine the taxonomic classifications, biological functions for ecological environment, and effects on human health.
Note: Our service is for research use only, and not for therapeutic or diagnostic use.
The various microbiome samples from indoor or outdoor environments. We have ever analyzed various samples collected from hospitals, schools, industrial buildings, etc.
Bacteria, fungi, virus.
Next-generation sequencing (NGS), long-read sequencing, PCR-DGGE, Real-time qPCR, anaerobic culture.
Illumina HiSeq/MiSeq, Ion PGM, PacBio SMRT systems, Nanopore systems, PCR-DGGE, Real-time qPCR, anaerobic culture.
Figure 1. High-throughput sequencing analysis process
Figure 2. PCR-DGGE analysis process
OTU Clustering | Distribution-Based OTU-Calling |
Rarefaction Curve | |
Shannon-Wiener Curve | |
Rank Abundance Curve | |
Diversity Index | |
OTU-Based Analysis | Heatmap |
VENN | |
Principal Components Analysis (PCA) | |
Hierarchical Clustering | |
Comparative Analysis | RDA/CCA |
PCA/PCoA | |
Network Analysis | |
Functional analysis | BLASTX, KEGG, eggNOG, CAZy, CARD, ARDB etc. |
PICRUSt | |
TAX4Fun | |
Phylogenetic Analysis | (Un)Weighted Unifrac |
Phylogenetic Trees |
Reference
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