DNA barcoding, a breakthrough technology, unveils the intricacies encoded within DNA sequences—highly conserved and genetically stable elements that exhibit variations across species and remain remarkably consistent within a species. This innovative biological identity system utilizes standardized, diverse, easily amplifiable, and short DNA fragments to deliver swift and self-initiated species identification.
At CD Genomics, we stand at the forefront of genetic exploration, offering a comprehensive suite of services that go beyond traditional sequencing and identification. Our cutting-edge short-read sequencing platform (Illumina and Sanger Sequencing) and long-read sequencing, coupled with an extensive understanding of diverse DNA barcoding primers, empower us to deliver unparalleled DNA barcode identification services.
Genomic DNA | ≥ 15 μL with a concentration between 50-100 ng/μL. OD260/280 for 1.8-2.2. Tested for undegraded DNA bands through 2% agarose gel electrophoresis is preferred. |
Plant material | Transport fresh tissues with leaves silica-dried, maintaining their integrity on dry ice for optimal results. For more details about the sample submission, please contact our technical team. |
Animal material | Preserve the essence of fresh tissues in anhydrous ethanol, transported at low temperatures to preserve the genetic richness. For more details about the sample submission, please contact our technical team. |
Deliverables | Raw data QC, FASTQ, BAM and VCF, etc. |
After sample reception, our meticulous workflow begins with the extraction of genomic DNA from the provided samples. Subsequently, this genomic DNA serves as the template for PCR amplification, utilizing specialized DNA barcode primers. The resulting PCR products undergo a purification process before advancing to the DNA sequencing phase.
Once the sequencing is completed, the spliced sequence files are subjected to a robust comparison using the NCBI Blast program, aligning the sequences with data stored in the extensive NCBI database. This critical step allows us to identify the species with the highest sequence similarity to the target species under investigation, thus delivering a precise and reliable identification result.
Our streamlined workflow combines cutting-edge technology, precise laboratory techniques, and a comprehensive database comparison to ensure the highest quality results for our clients.
CD Genomics DNA barcoding and specie identification service
We recognize the importance of enriching the collective knowledge base. We offer specialized plant and animal genome sequencing services. By doing so, we contribute to in-depth research, expanding and refining the barcode reference data for various species. Our goal is not just to provide data, but to empower your research with comprehensive genetic insights.
Types | Applications |
---|---|
DNA barcoding in plants |
|
DNA barcoding in animals |
|
Microbial DNA barcoding |
|
Environmental DNA metabarcoding |
|
For any general inquiries, please fill out the form below.
CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.