Sanger sequencing is the direct sequencing of PCR amplifications using primers designed for known target gene mutation sites. As the most accurate sequencing method, Sanger sequencing is widely used in plant and animal research for routine clone detection, single gene detection and small throughput screening, and because of its high precision and low cost, Sanger sequencing is widely used in plant and animal gene sequencing.
CD Genomics is committed to providing first-class, reliable Sanger sequencing services for agricultural science researchers. With our advanced sequencing service platform and professional technical team, we provide our customers with the highest quality and most reliable sequencing results. Since our establishment, we have provided tens of millions of routine sequencing services to customers dedicated to agrigenome research worldwide.
Fig 2. Sanger sequencing process.
Sample type | Concentration | Volume | Others |
---|---|---|---|
Plasmid | > 50 ng / µL | > 20 µL | Dissolution with double-distilled water. Large fragment plasmids need to specify vector length (>10 k). |
Purified PCR | > 10 ng / µL | > 20 µL | Single band detection by electrophoresis |
Bacteria | > 50 µL | Overnight culture of fresh bacterial broth. Indicate the resistance of the vector. Keep sealed to prevent cross-contamination or leakage. |
|
PCR stock solution | > 10 ng / µL | > 20 µL | Fragments usually larger than 200 bp, below 200 bp are recommended to be cloned and then sequenced. |
Primer | 10 µM (pmol / µL) | 5-10 µL / Each reaction |
While the advent of high-throughput sequencing technologies such as next- generation sequencing (NGS) has facilitated the massively parallel sequencing of DNA molecules, the robustness and accuracy (99.99%) offered by Sanger sequencing is still best suited for medium to low-throughput sequencing projects, and as the most used DNA sequencing technology worldwide, Sanger sequencing remains the gold standard for sequencing in most research applications. CD Genomics provides you with comprehensive and professional Sanger for your sequencing needs. If you are interested in us, feel free to contact us. We have professional technical services, experienced experts, and one-stop comprehensive services, start your project with us!
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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.