Sanger Sequencing Services

Sanger Sequencing Services

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Sanger sequencing services

Sanger sequencing is the direct sequencing of PCR amplifications using primers designed for known target gene mutation sites. As the most accurate sequencing method, Sanger sequencing is widely used in plant and animal research for routine clone detection, single gene detection and small throughput screening, and because of its high precision and low cost, Sanger sequencing is widely used in plant and animal gene sequencing.

CD Genomics is committed to providing first-class, reliable Sanger sequencing services for agricultural science researchers. With our advanced sequencing service platform and professional technical team, we provide our customers with the highest quality and most reliable sequencing results. Since our establishment, we have provided tens of millions of routine sequencing services to customers dedicated to agrigenome research worldwide.

Types of Sanger sequencing services

  • Animal and plant custom PCR services
    For customers with time-consuming and difficult to amplify target fragments, we provide optimized PCR services to help save your research time and improve research efficiency by providing you with custom PCR services according to your needs.
  • Animal and plant gene cloning and sequencing services
    One-stop, all-round gene cloning and sequencing services, we can construct your customized target genes in a short time, efficiently and accurately, and provide a full set of sequencing services to meet your any sequencing needs.
  • Microsatellite (SSR) analysis services
    Relying on our advanced sequencer detection platform, we specifically develop assays for SSR analysis to provide our customers with comprehensive SSR analysis services. Helping your research applications to obtain consistently accurate and reliable results.
  • Sanger sequencing of bacteria and phages
    Our company are committed to continuously optimizing sequencing processes to advance genomic research, reduce experimental processes, and improve experimental efficiency. Our services enable customers to directly sequence bacteria and phages without the need for plasmid preparation.

Sanger sequencing process

Sanger sequencing process-CD GenomicsFig 2. Sanger sequencing process.

Application of sanger sequencing in agriculture

  • Accelerate livestock breed improvement.
  • Improve animal and plant breeding.
  • Discovery of new plant species.
  • Validating the accuracy of gene editing technology in animals and plants.

Sample requirement

Sample type Concentration Volume Others
Plasmid > 50 ng / µL > 20 µL Dissolution with double-distilled water.
Large fragment plasmids need to specify vector length (>10 k).
Purified PCR > 10 ng / µL > 20 µL Single band detection by electrophoresis
Bacteria > 50 µL Overnight culture of fresh bacterial broth.
Indicate the resistance of the vector.
Keep sealed to prevent cross-contamination or leakage.
PCR stock solution > 10 ng / µL > 20 µL Fragments usually larger than 200 bp, below 200 bp are recommended to be cloned and then sequenced.
Primer 10 µM (pmol / µL) 5-10 µL / Each reaction

Service advantages and features

  • Advanced sequencing platform, professional technical team.
  • Deliver high quality and accurate sequencing results for you.
  • High speed, shortest delivery time, saving your research time.
  • Free universal primers, according to your needs to provide you with the most suitable sequencing solution.
  • Considerate service, any experimental problems, you can contact us at any time, we provide you with dedicated service throughout the process.

While the advent of high-throughput sequencing technologies such as next- generation sequencing (NGS) has facilitated the massively parallel sequencing of DNA molecules, the robustness and accuracy (99.99%) offered by Sanger sequencing is still best suited for medium to low-throughput sequencing projects, and as the most used DNA sequencing technology worldwide, Sanger sequencing remains the gold standard for sequencing in most research applications. CD Genomics provides you with comprehensive and professional Sanger for your sequencing needs. If you are interested in us, feel free to contact us. We have professional technical services, experienced experts, and one-stop comprehensive services, start your project with us!

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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.

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