Chloroplasts are the most important and ubiquitous plastids within plant cells and are the key site for photosynthesis. The chloroplast genome has a moderate rate of evolution, conserved and single-copy genes, higher resolution and better versatility than shorter DNA barcode sequences, so cpDNA sequencing can be applied to a variety of research areas such as plant species identification, phylogeny, revealing the origin of species, species evolution, and relatedness between different species. It also provides molecular evidence for the identification of new plant species.

CD Genomics relies on advanced sequencing platforms to provide comprehensive and in-depth analysis of plant chloroplast sequence information. We combine cpDNA sequencing results with knowledge from multiple disciplines to help agricultural science researchers analyze the origin and evolution of plant species, phylogenetic evolution, and genetic relationships through comparative analysis of plant chloroplast genomes.

Chloroplast DNA sequencing applications

• Plant species identification
• DNA barcode screening
• Phylogenetic analysis
• Chloroplast genetic engineering

Customer sample provision requirements

• DNA volume ≥ 1 μg
• DNA concentration ≥ 30 ng/μL

Chloroplast DNA sequencing process

Traditionally, cpDNA sequencing is mainly done by obtaining the chloroplast genome, using the conserved sequences of chloroplast genome to design simple primers, unknown sequences for PCR amplification, sequencing the amplification products for one generation, and finally splicing and assembling to get the complete chloroplast genome. This process is time-consuming.

CD Genomics keeps optimizing the experimental process and updating the sequencing technology, using high-throughput sequencing, isolating chloroplasts before extracting cpDNA, selecting appropriate chloroplast reference genomes, assembling these sequences, and finally obtaining the complete chloroplast genome sequencing results. In addition, our cpDNA sequencing technology is still effective for higher plant leaves where chloroplast isolation and cpDNA extraction are more difficult.

Fig 2. Chloroplast DNA sequencing process

Service features and advantages

• Comprehensive chloroplast DNA sequencing services. We provide a full range of sequencing services from cpDNA preparation to analysis of chloroplast sequencing results, helping our clients to shorten their research time.
• Cost-effective sequencing solutions. We customize the most cost-effective sequencing solutions according to customer needs, and our experts and technical team can deliver the sequencing results to customers in the shortest time.
• High-quality sequencing results. Our advanced sequencing platform and experienced professional sequencing team will provide you with accurate and error-free sequencing results.
• Thoughtful and comprehensive after-sales service. Reduce the worries of your project.

CD Genomics has rich experience in cpDNA sequencing service, so far we have provided cpDNA sequencing for multiple species worldwide, including not limited to strawberry, chrysanthemum, primrose, buckwheat, wheat, tomato and dozens of other species. Meanwhile, we will provide you with flexible cpDNA sequencing solutions according to your sequencing requirements. If you are interested in us, please feel free to contact us.