Sanger Sequencing Q&A

General Questions

The results of PCR product electrophoresis are only a rough qualitative result. Non-specific PCR amplification products that differ in size by only a few bases from the target fragment cannot be distinguished by the naked eye. However, Sanger DNA sequencing reactions are sensitive and objective, and can directly reflect the template itself. If you are not sure about the purity of the PCR product, we hope you can clone the PCR product to ensure good sequencing results.