What is Targeted DNA Methylation Sequencing

We can comprehend the methylation alterations in the genome better with the aid of targeted DNA methylation sequencing technique. Methylation is a frequent DNA change that can affect cell differentiation and gene expression. Therefore, it is essential to comprehend the methylation changes in the DNA when researching biology and disease causes.

Targeted DNA methylation sequencing technology is a powerful high-throughput method for rapid and precise detection of genome-wide methylation modifications. Leveraging the specificity of DNA methylation, this approach selectively enriches DNA fragments carrying methylation marks through the use of methylation-specific enzymes. These enriched fragments are subsequently subjected to high-throughput sequencing on specialized platforms, generating data of exceptional quality, and resolution.

This cutting-edge technology has revolutionized the field of biological and medical research. Its applications span diverse areas, including the investigation of tumor formation mechanisms and the identification of potential therapeutic targets. Furthermore, it plays a crucial role in epigenetics and developmental biology studies, unraveling the intricate regulatory networks governed by DNA methylation.

With its promise in shedding light on the complexities of genome-wide methylation modifications, targeted DNA methylation sequencing stands at the forefront of innovative sequencing methods, driving the quest for a deeper understanding of epigenetic processes and their implications in health and disease.

Advantages of Targeted DNA Methylation Sequencing

Targeted DNA methylation sequencing offers several significant advantages that make it a valuable tool in the field of epigenetics.

Firstly, the method employs a selective enrichment strategy for DNA fragments carrying methylation modifications. This targeted approach minimizes sequencing data noise and effectively reduces background interference, resulting in a cleaner and more accurate dataset. Researchers can thus confidently focus on the regions of interest and extract precise methylation information.

Secondly, the technology demonstrates a remarkable capability to detect low-frequency methylation modifications. This heightened sensitivity in detection allows researchers to uncover subtle changes in methylation patterns, even in regions with low levels of modification. Consequently, the method becomes especially advantageous in capturing rare epigenetic events that might play critical roles in various biological processes.

Furthermore, the versatility of targeted DNA methylation sequencing extends to the detection of different types of methylation modifications. By profiling CpG islands, non-CpG islands, and CHH methylation patterns, researchers gain a comprehensive understanding of the diverse landscape of epigenetic modifications in the genome. This broad coverage enhances the scope of investigation, revealing intricate relationships between methylation patterns and gene regulation.

Methodologies Included in Targeted DNA Methylation Analysis Service

1. EpiTYPER DNA Methylation Analysis

EpiTYPER is a high-throughput platform that utilizes base-specific cleavage and mass spectrometry to quantify DNA methylation levels at individual CpG sites. This method offers single nucleotide resolution, allowing researchers to precisely assess the methylation status of targeted regions.

2. NGS-BSP (Bisulfite Sequencing PCR)

NGS-BSP is a PCR-based approach that combines bisulfite treatment with PCR amplification to target specific genomic regions for methylation analysis. It is well-suited for investigating methylation patterns in multiple regions simultaneously.

3. Methyl seq

Methyl seq, or methyl capture sequencing, employs hybridization capture using pre-designed oligos to isolate DNA regions containing target CpG sites. This method enables the high-throughput analysis of multiple regions of interest in a single experiment.

4. CD Human Methylome Panel

The CD Human Methylome Panel is a pre-validated, off-the-shelf panel specifically designed to assess the methylation levels of genes associated with cancer, pluripotency, and other biological processes.

5. Targeted Bisulfite Sequencing

Targeted Bisulfite Sequencing focuses on selective regions of interest, allowing researchers to obtain detailed methylation patterns with single nucleotide resolution. This method is ideal for hypothesis-driven studies and biomarker validation.

Advantages of Targeted DNA Methylation Analysis Service

Cost-Effectiveness and Faster Turnaround Time: By focusing on specific genomic regions of interest, this targeted approach reduces the overall cost of sequencing and data analysis. It also significantly shortens the turnaround time, allowing researchers to obtain high-quality methylation data more efficiently.

Enhanced Accuracy, Sensitivity, and Specificity: Targeted DNA Methylation Analysis provides higher coverage and deeper sequencing of CpG sites in the selected regions. This results in enhanced accuracy, sensitivity, and specificity in identifying methylation changes, even in low-frequency events.

Reduced Data Complexity: By analyzing only the regions of interest, researchers can manage and interpret the data more effectively, leading to streamlined data analysis and more straightforward interpretation of results.

Suitable for Hypothesis Testing and Biomarker Validation: This service is particularly valuable for hypothesis-driven studies where researchers want to investigate the methylation patterns of specific target regions. Additionally, it is an excellent tool for validating potential epigenetic biomarkers associated with diseases or other biological processes.

Data Analysis

CD Genomics' Targeted DNA Methylation Analysis Service offers a powerful and comprehensive solution for studying DNA methylation patterns with high precision and sensitivity. With a focus on specific genomic regions, this service provides researchers with valuable insights into the epigenetic regulation of genes and its impact on various biological processes. The advanced data analysis options, in combination with various cutting-edge methodologies, make this service a valuable resource for both hypothesis-driven research and biomarker validation studies.