PacBio HiFi sequencing variant detection supports all species. The reason why the current results are currently still mainly human, this is mainly because the current human reference genome is currently the most accurate.
Generally speaking, it depends on how many ploidy the specific assembled genome is. Take the human genome for example, we recommend a minimum of 15X-20X, if the genome is more than one haplotype need to increase the data of 10X HiFi. But it also depends on the complexity of the genome, if it is a complex genome may require an additional 20X HiFi data for one more haplotype.
We have only tested insects and arthropods for the time being, because the DNA content of individual individuals of these samples are very small. Microorganisms we have not tested for now. To clarify, the genome is less than 500M, this is for De Novo application, if it is resequencing there is no genome size limit.
This is mainly related to genomic heterozygosity. When assembling, if the difference between two haplotypes is large, then the difference will be reflected in the assembly result, and it will look larger.
We use long range PCR. PacBio has done a lot of tests to select the two PCR enzymes that can better cover the genomic regions with high GC and low GC as much as possible. After sample interruption, we split the sample into two parts, one for PCR A and the other for PCR B. The instrument is able to obtain the most uniform genomic coverage.
For Research Use Only. Not for use in diagnostic procedures.