HiSeq X Ten

Through continuous innovation, Illumina launched a new system-the HiSeq X Ten System, a set of 10 HiSeq X instruments. Each HiSeq X System can sequence a human genome at 30x coverage, and it is the first and only platform to deliver a $1000 human genome, generating tens of thousands of high-quality, high-coverage genome sequences. High throughput is largely based on advanced Patterned Flow Cell Design—Patterned flow cells contain billions of nanowells at fixed locations providing even cluster spacing and uniform feature size to deliver extremely high cluster density.

With its ultrahigh throughput and unprecedented low price per genome, the HiSeq X Ten System makes population-scale whole-genome sequencing (WGS) a reality.

Moreover, HiSeq X Systems can now apply population-scale sequencing to nonhuman species, which delivers high coverage in various areas, including agriculture and model organism research in the pharmaceutical industry.

By providing ultra-high–throughput and an unprecedented price point per genome, the HiSeq X System provides customers with an opportunity to reshape the economics and scale of whole-genome sequencing.

Highlights:

• Reliable performance, high data quality
• Excellent library preparation
• High-throughput sequencing capability
• Low cost, high operational efficiency
• Fast, accurate WGS analysis
• Suitable for human population studies
• Wide range of applications

Applications:

Population-scale Projects: The HiSeq X Ten platform stands as an optimal choice for extensive population studies with the objective of deciphering genotypic variability and its repercussions on human health. Its utility lies in facilitating the scrutiny of genetic diversity spanning both coding and non-coding regions, thereby furnishing invaluable insights into the realms of human biology and genetic ailments.

Clinical Genomics: This cutting-edge platform expedites the integration of clinical genomics and the advent of next-generation medical practices by enabling exhaustive genomic evaluations on a populace-wide scale. It nurtures collaborative endeavors among governmental bodies, industrial entities, and medical research stakeholders, thereby propelling advancements in healthcare.

Whole Genome Sequencing (WGS): The HiSeq X Ten platform is renowned for its exceptional throughput and efficiency, making it adept at rapidly conducting large-scale whole genome sequencing initiatives. This sequencing methodology provides a comprehensive profile of genomic information, spanning genomic architecture, SNPs, insertions, deletions, and other genetic variants. Consequently, it boasts a wide array of applications in genetic research, population genetics, and the identification of disease-associated genes.

Exome Sequencing: In the realm of exome sequencing projects, the HiSeq X Ten platform delivers high coverage and precise data, facilitating the identification of variations within exonic regions, including pathogenic mutations and potential disease-linked genes. This sequencing approach holds profound significance in genetic disease investigation, oncology research, and the exploration of drug targets.

RNA Sequencing: Leveraging the high-throughput capabilities of the HiSeq X Ten platform, we facilitate a range of transcriptome sequencing capabilities encompassing mRNA, microRNA, and full-length RNA sequencing. This technique unfolds gene expression patterns, pinpoints differential expression genes, and dissects their functional roles and regulation in various biological phenomena.

DNA Methylation Sequencing: The HiSeq X Ten platform is also proficiently deployed for DNA methylation sequencing. As a pivotal instrument in studying epigenetic regulatory frameworks and assessing the influence of environmental factors on genome methylation, this high-throughput sequencing modality permits an all-inclusive comprehension of genomic methylation levels and the examination of their correlations with disease manifestation and progression.

Single-cell Sequencing: Thanks to its high throughput and sensitivity, the HiSeq X Ten platform is aptly suited for single-cell sequencing investigations. This intriguing facet of sequencing underscores the diversity of cell types, highlights the heterogeneity of phenotypes, and demystifies transcriptomic variations between individual cells. This approach possesses profound implications in the progression of biomedical and life sciences research.