Microbiome Analysis Services

Microorganisms are widely distributed in every ecosystem on the earth and play an important role in the material cycle of carbon, nitrogen and sulfur. With the development of bioinformatics and the integrated application of multi-omics, systematic research on the function, metabolism, and community structure of microorganisms has become more and more in-depth.

Microbial 16S/18S/ITS sequencing service

The 16S rDNA sequence contains 9 variable regions and 10 conserved regions. The variable region differs from bacteria to bacteria, and the degree of variation is closely related to the phylogeny of bacteria. By detecting the sequence variation and abundance of 16S rDNA, we can understand the community diversity information in environmental samples. The analysis based on 16S rDNA plays an important role in the classification and identification of microorganisms and micro-ecological research.

18S rDNA or ITS (Internal Transcribed Spacer) is widely used in the classification and identification of eukaryote and fungi. 18S rDNA is more suitable for the classification of elements above species level in phylogenetic research. ITS sequences are located between 18S, 5.8S and 28S rRNA genes of fungi, which are ITS 1 and ITS 2, respectively. ITS belongs to a moderately conservative region, and it can be used to study species and classification levels below species.

CD Genomics can provide you with 16S/18S/ITS and other microbial species characteristic sequence amplification and high-throughput sequencing as well as professional biological information analysis.

• Bioinformatics analysis

OTU clustering and analysis
Species classification
Single sample diversity analysis (alpha diversity)
Comparative analysis of diversity among samples (beta diversity)
Species difference analysis
Function analysis: KEGG function prediction, COG function prediction
Association analysis and model prediction

Microbial metagenomic sequencing

Metagenomic analysis takes the genomes of all microorganisms in the environment as the research object, and obtains the saturated data amount of a single sample through high-throughput sequencing of whole genomic DNA in environmental samples. Based on de novo assembly, analyze the diversity of microbial community structure, the genetic composition and function of microbial populations, and the metabolic pathways related to specific environments.

• Bioinformatics analysis

Gene prediction and gene set construction
Genetic analysis: Venn diagram and difference analysis
Gene set function annotation: Eggnog, KEGG, CARD, COG, CAZy, Swiss-prot, NR, etc.
Species analysis
Functional analysis
Diversity analysis
Network interaction
Similarity analysis
Association analysis and model prediction

Microbial metabolomics analysis

Targeted metabolomics analysis: identification and quantitative analysis of target small molecules, including microbial primary metabolites and secondary metabolites.

Untargeted metabolomics analysis: unbiased detection of the dynamic changes of all small molecule metabolites before and after being stimulated or disturbed, and screening of differential metabolites through biometric analysis, and pathway analysis of differential metabolites.

• Bioinformatics Analysis

Classification annotation and functional annotation analysis of metabolites
Screening of differential metabolites (PCA analysis, PLS-DA, etc.)
Differential metabolite analysis

Microbial proteomics analysis

CD Genomics has established an accurate, sensitive, and high-throughput microbial proteome analysis platform to study the characteristics of proteins, including protein expression levels, post-translational modifications, and protein-protein interactions.

• Bioinformatics Analysis

  Functional annotation (GO, KEGG, COG)
  Statistical analysis (volcano plot, veen plot, etc.)
  Enrichment analysis (GO, KEGG)
  Clustering analysis
  Protein-protein interactions analysis

CD Genomics can provide you with microbiome analysis services and high-quality result reports. If you have any questions about the above services or consult other microbiome analysis services, please contact us.

*For Research Use Only. Not for use in diagnostic procedures.