LcWGS is a technology based on Next-generation sequencing (NGS) technology, with a sequencing depth of less than 10X (generally 0.5X-6X in real application scenarios), to sequence the entire DNA sequences of individual genomes of existing reference genomes and to analyze the variability of individuals or groups. Compared with conventional WGS technology, LcWGS only adds an extra step of genotype filling in the analysis, but it can realize the identification and typing of genome-wide genetic variants at a lower sequencing depth and cost than conventional WGS.
Based on our next-generation sequencing platform, CD Genomics has developed a full set of LcWGS and low-coverage resequencing analysis processes for agricultural plants and animals, providing GWAS, genetic structure analysis of economically important traits, and a theoretical basis for researching new genetic analysis methods and breeding.
You can refer to our Genotyping By Sequencing (GBS) platforms for more information.
When selecting between LC-WGS, WGS, GBS, and Microarrays, consider the following factors:
Technology | Initial Investment | Operational Cost | Flexibility | Operational Complexity | Reference Required | Discovery Potential |
---|---|---|---|---|---|---|
WGS (Whole Genome Sequencing) | None | Poor | Good | Good | Good | Good |
GBS (Genotyping-by-Sequencing) | Poor | Good | Fair | Good | Poor | Fair |
Low Coverage Sequencing | None | Good | Good | Good | Fair | Good |
Microarrays | Poor | Good | Fair | Good | Poor | Poor |
Select the technology aligned with your research goals, budget constraints, and the level of genomic detail required. If you have more questions, please contact our technique team.
Sample type | Genomic DNA |
Sample concentration | ≥ 20 ng/μL |
Total sample | ≥ 500 μg |
Sample purity | OD260/280 for 1.8-2.2 |
Electrophoresis requirements | Clear main band, no degradation or mild degradation |
Deliverables | Raw data QC, FASTQ, BAM and VCF, etc. |
1. Genome-Wide Association Studies (GWAS)
Uncover the genetic basis of complex traits and diseases through our LC-WGS, enabling robust GWAS for a deeper understanding of the underlying genomic architecture.
2. Key Trait Genetic Structure Analysis
Gain insights into the genetic foundations of economically significant traits in agricultural plants and animals, facilitating informed breeding strategies.
Explore the intricate dynamics of population genetics, enabling comprehensive studies on diversity, migration, and adaptation.
4. Novel Genetic Analysis Methods
Pioneer research in innovative genetic analysis methods, leveraging the extensive data provided by LC-WGS to push the boundaries of genetic exploration.
Provide a theoretical framework for advanced breeding practices by understanding the genetic landscape and variability within a population.
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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.