Long Read Sequencing for Plant and Animal Genetic Engineering

Advances in genetic engineering have revolutionized the fields of agriculture and animal husbandry, providing unprecedented opportunities to improve crop traits, increase livestock productivity, and address global challenges such as food security and sustainability. In order to be able to write new genomes, scientists must be able to read them quickly and reliably. CD Genomics, a leading company with extensive experience in genomics research, is committed to providing long-read sequencing solutions for plant and animal genetic engineering.

Introduction to Plant and Animal Genetic Engineering

Genetic engineering (also known as genetic modification) is the process of altering the composition of an organism's DNA using laboratory-based techniques. This may involve changing individual base pairs (AT or CG), deleting DNA regions, or adding new DNA fragments. For example, genetic engineering may involve adding genes from one species to an organism of a different species to produce a desired trait. Advances in genome engineering and other programmable molecular technologies show great promise in the field of agriculture, with the potential to address issues of food security and industrial sustainability. One of the key factors in realizing these breakthroughs is accurate and comprehensive sequencing of plant and animal genomes. High-fidelity sequencing can help you validate where to make edits on a genome-wide or target basis. It can also help you design more efficient workflows with extremely accurate genomic information.

Fig. 1. CRISPR-Cas9 targeted cleavage for the isolation of a region of interest, followed by enrichment and LRS using ONT. (López-Girona et al., 2020)

Solutions Offering at CD Genomics

CD Genomics offers cutting-edge long read sequencing solutions tailored to plant and animal genetic engineering projects. Our state-of-the-art sequencing technology combined with our highly skilled team of scientists and bioinformaticians enables researchers and breeders to realize the full potential of their efforts.

CD Genomics provides genomic information for precise genomic modifications and realization of desired traits in plant and animal genetic engineering programs. Using highly accurate long read sequencing data ensures that you obtain the detailed sequence information needed for the most complex gene editing programs, including creating high-quality reference genomes, finding splice variants within the transcriptome, validating edited sites, capturing on- and off-target effects, and identifying structural rearrangements on chromosomes.

Applications of Our Long Read Sequencing Solutions for Plant and Animal Genetic Engineering

• Cassava Genetic Engineering

Cassava is a multipurpose food crop and one of the five most important sources of carbohydrates globally. The vegetable is difficult to genetically transform and regenerate in vitro, and exhibits poor fertility in some breeding lines and farmer-preferred varieties. Even traditional breeding programs used for trait infiltration are very time-consuming and flowering rarely occurs in greenhouse environments, with sexual reproduction producing few seeds. We provide by long read sequencing to track changes in modified genes, such as deletions, insertions, and shifted-code mutations, to better understand their genomic and phenotypic characteristics.

• Cow Genetic Engineering

We offer long read sequencing to help you create well-designed domesticates without relying on viruses and bacteria to deliver the DNA. Our long read sequencing can be used to generate reference assemblies that include the complete bovine genome as well as plasmid backbones and template sequences.

Advantages of Long Read Sequencing Solutions for Plant and Animal Genetic Engineering

• Long read sequencing enhances genome assembly and structural variation detection. This information is critical for understanding the genetic basis of phenotypic traits and designing precise genetic modifications.
• Long read sequencing enables accurate resolution and annotation of repetitive regions.
• Long read sequencing accurately captures full-length mRNA molecules for intensive transcriptome analysis, enhancing the understanding of gene function libraries and their potential role in trait development.

With the benefits of long read sequencing, including comprehensive genome coverage, accurate identification of structural variation, facilitation of complex genome assemblies, and enhanced validation of genome editing, researchers can gain a deeper understanding of the genetic engineering of plants and animals. If you have any questions, please feel free to contact us. We look forward to working with you on projects of interest.

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