CRISPR/Cas9 is a gene editing tool that enables the treatment of complex diseases, rare diseases and genetic disorders through DNA splicing technology. CRISPR/Cas9 is an adaptive immune defense developed by bacteria and archaea during their long evolution and can be used against invading viruses and exogenous DNA. The main principle of CRISPR/Cas9 gene editing technology is to genetically modify the targeted genes with specific DNA modifications.
Figure 1. Schematic diagram of CRISPR/Cas9 gene editing system
CRISPR screening is an important method for functional target screening, and CD Genomics utilizes high-throughput NGS for efficient CRISPR screening, which is very cost effective and highly accurate. All CRISPR-related sequencing services are maintained under strict QC standards. With the support of CD Genomics' sequencing services, you are sure to be inspired and inspired to do more gene editing related research.
CD Genomics is committed to supporting biopharmaceutical and life science companies in their research on novel gene therapies through NGS-based CRISPR sequencing-related services. CD Genomics offers gene editing solutions that focus on CRISPR screening sequencing, CRISPR validation sequencing and CRISPR off-target assays.
At CD Genomics, NGS-based high-throughput strategies can support your CRISPR screening process, enabling efficient, high-quality screening and analysis of targeted genes. We offer a standardized sequencing process including sgRNA library design and synthesis, lentiviral vector construction and infection of host cells, phenotype selection, high-throughput sequencing and bioinformatics analysis. Rigorous QC characterization ensures the accuracy and comprehensiveness of your analysis reports.
The validation of target activity and whether it is correctly edited after CRISPR/Cas9 genome editing is very important. With NGS-based high-throughput tools, CD Genomics can provide you with efficient and accurate validation of gene editing results. The entire process involves our experienced experts and is maintained under strict quality control standards. CRISPR validation is an important guide for gene editing and target screening, and the main purpose is to help you analyze target activity and predict editing effects.
CD Genomics provides you with high-throughput NGS-based tools to identify and detect off-target sites, helping you optimize your gene editing strategy and reduce the occurrence of off-target effects. When off-targeting occurs in CRISPR, it can lead to mis-editing of genes and the consequences will be very serious. Not only will this not achieve the original purpose of gene editing, but it will also have huge side effects. Gene editing only makes sense if the target gene is correctly targeted, and detecting off-target effects is the most important step in developing a gene editing strategy. The importance of off-target detection directly determines the success of gene editing. The whole process is operated acutely by a professional team of experts under strict QC standards.
We provide global off-target site detection services based on next-generation sequencing platforms. Our platform is a genome-wide method for identifying off-target effects through high-throughput sequencing technology, and it is quite sensitive, maintaining high sensitivity for low-frequency mutation detection efficiency with low transfection efficiency, and improving detection accuracy.
We are convinced that CD Genomics is the partner you have been seeking for your genome editing and engineering. Our automated sequencing platform and dedicated team of experts guarantee the accuracy of sequencing and analysis results. If you are interested in the genome editing and engineering solutions offered by CD Genomics, please feel free to contact us.
CD Genomics is transforming biomedical potential into precision insights through seamless sequencing and advanced bioinformatics.
