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CD Universal Blocking Oligo Kit For Illumina

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PN004-01 5
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CD Universal Blocking Oligo Kit For Illumina is a single reagent, designed for use with Illumina dual- or single-indexed adapters in hybridization capture-based target enrichment experiments, to improve on-target performance by reducing adapter-to-adapter hybridization. CD Genomics provide Ready-to-Use universal blocking oligo for Illumina TruSeq librar. Besides, we offer one-on-one service to help you customize the exclusive blocking oligos for specialized applications or other sequencing platforms.

CD Universal Blocking Oligo Kit For Illumina is a single reagent intended to optimize on-target performance by lessening adapter-to-adapter hybridization in hybridization capture-based target enrichment experiments using Illumina dual- or single-indexed adapters. In comparison to experiments without blocking oligos, incorporation of blocking oligos in the hybridization step prevents adapter-adapter binding and increases on-target mapped reads. Since this kit eliminates the need to match individual blocking oligos to specific adapter sequences, it allows for a more efficient workflow.

Application:

Blocking free adapters from the library pool by Blocking Oligo can reduce index hopping rates and the possibility of misalignment, which can be used to prevent adapter-adapter binding and increase the on-target mapped reads.

Storage:

All the components should be stored at -20℃.

Specifications:

Features Improve on-target performance by reducing adapter-to-adapter hybridization;cost-effective.
Application It is recommended to use this reagent in hybridization capture-based target enrichment experiments.
Sequencing Platform Illumina

  1. Centrifuge tubes before opening. Some of the product may have been dislodged during shipping.
  2. Resuspend CD Universal Blocking Oligos to 1μL/reaction. The volume of the solution added is according to the product size of the kit.
  3. Mix thoroughly by pipetting up and down several times. Centrifuge briefly to collect all the resuspended material at the bottom of the tube. Note: Store resuspended oligos at –20°C.

* For Research Use Only. Not for use in diagnostic procedures.

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