CD ssDNA Index Kit for Illumina, Adapter for Illumina

CAT	Specs	Inquiry	Basket
DSI003-01	48
DSI003-02	48

The CD ssDNA Index Kit for Illumina was created specifically for the Illumina CD ssDNA-seq Lib Prep Kit and CD Methyl-DNA Lib Prep Kit. The kit includes two kits for preparing DNA libraries for Illumina platforms. A Universal PCR Primer and 48 different 8bp indexed PCR primers are included in each kit. Different specimens can be distinguished using single-indexed primers. The kit is ideal for creating NGS libraries from denatured DNA, viral DNA, ancient DNA that has been highly degraded, and other single-stranded DNA samples. The ssDNA kit's workflow is straightforward: make libraries in two steps, then PCR and cleanup.

Components:

Components of CD ssDNA Index Kit for Illumina

Application:

Special for CD ssDNA-seq Lib Prep Kit for Illumina (DP003) and CD Methyl-DNA Lib Prep Kit for Illumina (EP005).

Storage:

All the components can be stored at -20℃ for one year.

Components:

CD ssDNA Index Kit for Illumina-Components

Specifications:

Application	Used for : CD ssDNA-seq Lib Prep Kit for Illumina CD Methyl-DNA Lib Prep Kit for Illumina
Sequencing Platform	Illumina

Related Products

Universal PCR Primer:

5'-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'

Index Primer:

5'-CAAGCAGAAGACGGCATACGAGAT[index]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3'

The Index name corresponding to each primer, the Index sequence included in the primer as follows:

DSI003-01

Index Name

Index Sequence

Index Name

Index Sequence

Index Name

Index Sequence

PCR Index1

TTACCGAC

PCR Index17

AGGATAGC

PCR Index33

TGGAAGCA

PCR Index2

AGTGACCT

PCR Index18

CCTTCCAT

PCR Index34

AGCTAAGC

PCR Index3

TCGGATTC

PCR Index19

GTCCTTGA

PCR Index35

GAACGGTT

PCR Index4

CAAGGTAC

PCR Index20

TGCGTAAC

PCR Index36

GGAATGTC

PCR Index5

TCCTCATG

PCR Index21

CACAGACT

PCR Index37

TACGGTCT

PCR Index6

GTCAGTCA

PCR Index22

TTACGTGC

PCR Index38

CCAGTATC

PCR Index7

CGAATACG

PCR Index23

CCAAGGTT

PCR Index39

TCTACGCA

PCR Index8

TCTAGGAG

PCR Index24

CACGCAAT

PCR Index40

GTAACCGA

PCR Index9

CGCAACTA

PCR Index25

TTCCAGGT

PCR Index41

GACGTCAT

PCR Index10

CGTATCTC

PCR Index26

TCATCTCC

PCR Index42

CTTACAGC

PCR Index11

GTACACCT

PCR Index27

GAGAGTAC

PCR Index43

TCCATTGC

PCR Index12

CGGCATTA

PCR Index28

GTCGTTAC

PCR Index44

AGCGAGAT

PCR Index13

TCGTCTGA

PCR Index29

GGAGGAAT

PCR Index45

CAATAGCC

PCR Index14

AGCCTATC

PCR Index30

AGGAACAC

PCR Index46

AAGACACC

PCR Index15

CTGTACCA

PCR Index31

CAGTGCTT

PCR Index47

CCAGTTGA

PCR Index16

AGACCTTG

PCR Index32

CTTGCTAG

PCR Index48

TGGTGAAG

DSI003-02

Index Name

Index Sequence

Index Name

Index Sequence

Index Name

Index Sequence

PCR Index49

AAGACCGT

PCR Index65

GACGAACT

PCR Index81

AGCTACCA

PCR Index50

TTGCGAGA

PCR Index66

AGACGCTA

PCR Index82

CTTCACTG

PCR Index51

GCAATTCC

PCR Index67

ATAACGCC

PCR Index83

GGTTGAAC

PCR Index52

GAATCCGT

PCR Index68

GAATCACC

PCR Index84

GATAGCCA

PCR Index53

CCGCTTAA

PCR Index69

GGCAAGTT

PCR Index85

TACTCCAG

PCR Index54

TACCTGCA

PCR Index70

GATCTTGC

PCR Index86

GGAAGAGA

PCR Index55

GTCGATTG

PCR Index71

CAATGCGA

PCR Index87

GCGTTAGA

PCR Index56

TATGGCAC

PCR Index72

GGTGTACA

PCR Index88

ATCTGACC

PCR Index57

CTCGAACA

PCR Index73

TAGGAGCT

PCR Index89

AACCAGAG

PCR Index58

CAACTCCA

PCR Index74

CGAATTGC

PCR Index90

GTACCACA

PCR Index59

GTCATCGT

PCR Index75

GTCCTAAG

PCR Index91

GGTATAGG

PCR Index60

GGACATCA

PCR Index76

CTTAGGAC

PCR Index92

CGAGAGAA

PCR Index61

CAGGTTCA

PCR Index77

TCCACGTT

PCR Index93

CAGCATAC

PCR Index62

GAACGAAG

PCR Index78

CAACACAG

PCR Index94

CTCGACTT

PCR Index63

CTCAGAAG

PCR Index79

GCCTTAAC

PCR Index95

CTTCGGTT

PCR Index64

CATGAGCA

PCR Index80

GTAAGGTG

PCR Index96

CCACAACA

Quality Control
16-Hour Incubation: A 50 ul reaction system containing 5 ul of Oligos and 1 μg of Hind III-λ DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis. A 50 μl reaction system containing 5 ul of Oligos and 1 μg of T3 DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis.
Endonuclease Activity: A 50 ul reaction system containing 5 ul of DNA adapters and 1 ug of pX174RF I DNA incubated at 37°C for 4 hours resulted in < 10% conversion to RF Il analyzed by agarose gel electrophoresis.

Index Name	Index Sequence	Index Name	Index Sequence	Index Name	Index Sequence
PCR Index1	TTACCGAC	PCR Index17	AGGATAGC	PCR Index33	TGGAAGCA
PCR Index2	AGTGACCT	PCR Index18	CCTTCCAT	PCR Index34	AGCTAAGC
PCR Index3	TCGGATTC	PCR Index19	GTCCTTGA	PCR Index35	GAACGGTT
PCR Index4	CAAGGTAC	PCR Index20	TGCGTAAC	PCR Index36	GGAATGTC
PCR Index5	TCCTCATG	PCR Index21	CACAGACT	PCR Index37	TACGGTCT
PCR Index6	GTCAGTCA	PCR Index22	TTACGTGC	PCR Index38	CCAGTATC
PCR Index7	CGAATACG	PCR Index23	CCAAGGTT	PCR Index39	TCTACGCA
PCR Index8	TCTAGGAG	PCR Index24	CACGCAAT	PCR Index40	GTAACCGA
PCR Index9	CGCAACTA	PCR Index25	TTCCAGGT	PCR Index41	GACGTCAT
PCR Index10	CGTATCTC	PCR Index26	TCATCTCC	PCR Index42	CTTACAGC
PCR Index11	GTACACCT	PCR Index27	GAGAGTAC	PCR Index43	TCCATTGC
PCR Index12	CGGCATTA	PCR Index28	GTCGTTAC	PCR Index44	AGCGAGAT
PCR Index13	TCGTCTGA	PCR Index29	GGAGGAAT	PCR Index45	CAATAGCC
PCR Index14	AGCCTATC	PCR Index30	AGGAACAC	PCR Index46	AAGACACC
PCR Index15	CTGTACCA	PCR Index31	CAGTGCTT	PCR Index47	CCAGTTGA
PCR Index16	AGACCTTG	PCR Index32	CTTGCTAG	PCR Index48	TGGTGAAG