CD RNA Adapters S3-S6 for Illumina, Adapter for Illumina

CAT	Specs	Inquiry	Basket
RSI002-03	192
RSI002-04	192
RSI002-05	192
RSI002-06	192

CD RNA Adapters S3-S6 for Illumina are designed specifically for the preparation of CD RNA libraries on Illumina platforms. There are four kits in the kit and each kit includes a Universal Adapter, as well as 24 different 8bp, indexed RNA Adapters that can be used to differentiate between samples. It is a dedicated kit for Illumina high-throughput sequencing platform library construction, ideal for Illumina high-throughput sequencing platform multi-sample RNA library construction.

Components:

Components of CD RNA Adapters S3-S6 for Illumina

Application:

Special for RNA library preparation for Illumina platforms with CD mRNA-seq Library Prep Kit for Illumina (RP001), CD Stranded mRNA-seq Library Prep Kit for Illumina (RP002) or CD Total RNA-seq Library Prep Kit for Illumina(human/mouse/rat) (RP003).

Storage:

All the components can be stored at -20℃ for one year.

Specifications:

Application	Used for: CD mRNA-seq Library Prep Kit for Illumina CD Stranded mRNA-seq Library Prep Kit for Illumina CD Total RNA-seq Library Prep Kit for Illumina(human/mouse/rat)
Sequencing Platform	Illumina

The structure of libraries prepared with CD RNA Adapters s3-s6 for Illumina are as follows:

5’ - Universal Adapter - Insert RNA Sequence - DNA Adapter 96-X - 3’

Both the Universal Adapter and the Index are contained in each DNA Adapter. The sequences of RNA Adapters are as follows:

Name	Sequence
Universal Adapter	5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
RNA Adapter 96-X	5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACIIIIIIIIATCTCGTATGCCGTCTTCTGCTTG-3’

IIIIIIII indicates the index sequences (8 bp). Please input the related Index sequences of RNA Adapters to the Sample Sheet before sequencing as follows:

RSI002-03	Index	RSI002-04	Index	RSI002-05	Index	RSI002-06	Index
RNA Adapter 96-01	AACGTGAT	RNA Adapter 96-25	AGATCGCA	RNA Adapter 96-49	GATAGACA	RNA Adapter 96-73	AATGTTGC
RNA Adapter 96-02	AAACATCG	RNA Adapter 96-26	AGCAGGAA	RNA Adapter 96-50	GCCACATA	RNA Adapter 96-74	ACACGACC
RNA Adapter 96-03	ATGCCTAA	RNA Adapter 96-27	AGTCACTA	RNA Adapter 96-51	GCGAGTAA	RNA Adapter 96-75	ACAGATTC
RNA Adapter 96-04	AGTGGTCA	RNA Adapter 96-28	ATCCT GTA	RNA Adapter 96-52	GCTAACGA	RNA Adapter 96-76	AGATGTAC
RNA Adapter 96-05	ACCACTGT	RNA Adapter 96-29	ATTGAGGA	RNA Adapter 96-53	GCTCGGTA	RNA Adapter 96-77	AGCACCTC
RNA Adapter 96-06	ACATTGGC	RNA Adapter 96-30	CAACCACA	RNA Adapter 96-54	GGAGAACA	RNA Adapter 96-78	AGCCATGC
RNA Adapter 96-07	CAGATCTG	RNA Adapter 96-31	GACTAGTA	RNA Adapter 96-55	GGTGCGAA	RNA Adapter 96-79	AGGCTAAC
RNA Adapter 96-08	CATCAAGT	RNA Adapter 96-32	CAATGGAA	RNA Adapter 96-56	GTACGCAA	RNA Adapter 96-80	ATAGCGAC
RNA Adapter 96-09	CGCTGATC	RNA Adapter 96-33	CACTTCGA	RNA Adapter 96-57	GTCGTAGA	RNA Adapter 96-81	ATCATTC C
RNA Adapter 96-10	ACAAGCTA	RNA Adapter 96-34	CAGCGTTA	RNA Adapter 96-58	GTCTGTCA	RNA Adapter 96-82	ATTGGCTC
RNA Adapter 96-11	CTGTAGCC	RNA Adapter 96-35	CATACCAA	RNA Adapter 96-59	GTGTTCTA	RNA Adapter 96-83	CAAGGAGC
RNA Adapter 96-12	AGTACAAG	RNA Adapter 96-36	CCAGTTCA	RNA Adapter 96-60	TAGGATGA	RNA Adapter 96-84	CACCTTAC
RNA Adapter 96-13	AACAACCA	RNA Adapter 96-37	CCGAAGTA	RNA Adapter 96-61	TATCAGCA	RNA Adapter 96-85	CCATCCTC
RNA Adapter 96-14	AACCGAGA	RNA Adapter 96-38	CCGTGAGA	RNA Adapter 96-62	TCCGTCTA	RNA Adapter 96-86	CCGACAAC
RNA Adapter 96-15	AACGCTTA	RNA Adapter 96-39	CCTCCTGA	RNA Adapter 96-63	TCTTCACA	RNA Adapter 96-87	CCTAATCC
RNA Adapter 96-16	AAGACGGA	RNA Adapter 96-40	CGAACTTA	RNA Adapter 96-64	TGAAGAGA	RNA Adapter 96-88	CCTCTATC
RNA Adapter 96-17	AAGGTACA	RNA Adapter 96-41	CGACTGGA	RNA Adapter 96-65	TGGAACAA	RNA Adapter 96-89	CGACACAC
RNA Adapter 96-18	ACACAGAA	RNA Adapter 96-42	CGCATACA	RNA Adapter 96-66	TGGCTTCA	RNA Adapter 96-90	CGGATTGC
RNA Adapter 96-19	ACAGCAGA	RNA Adapter 96-43	CTCAATGA	RNA Adapter 96-67	TGGTGGTA	RNA Adapter 96-91	CTAAGGTC
RNA Adapter 96-20	ACCTCCAA	RNA Adapter 96-44	CTGAGCCA	RNA Adapter 96-68	TTCACGCA	RNA Adapter 96-92	GAACAGGC
RNA Adapter 96-21	ACGCTCGA	RNA Adapter 96-45	CTGGCATA	RNA Adapter 96-69	AACTCACC	RNA Adapter 96-93	GACAGTGC
RNA Adapter 96-22	ACGTATCA	RNA Adapter 96-46	GAATCTGA	RNA Adapter 96-70	AAGAGATC	RNA Adapter 96-94	GAGTTAGC
RNA Adapter 96-23	ACTATGCA	RNA Adapter 96-47	CAAGACTA	RNA Adapter 96-71	AAGGACAC	RNA Adapter 96-95	GATGAATC
RNA Adapter 96-24	AGAGTCAA	RNA Adapter 96-48	GAGCTGAA	RNA Adapter 96-72	AATCCGTC	RNA Adapter 96-96	GCCAAGAC

Quality Control

16-Hour Incubation: A 50 ul reaction system containing 5 ul of Oligos and 1 μg of Hind III-λ DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis. A 50 μl reaction system containing 5 ul of Oligos and 1 μg of T3 DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis.

Endonuclease Activity: A 50 ul reaction system containing 5 ul of RNA adapters and 1 ug of pX174RF I DNA incubated at 37°C for 4 hours resulted in < 10% conversion to RF Il analyzed by agarose gel electrophoresis.