CD DNA Adapters S3-S6 for Illumina, Adapter for Illumina

CAT	Specs	Inquiry	Basket
DSI002-03	192
DSI002-04	192
DSI002-05	192
DSI002-06	192

The Illumina DNA Adapters S3-S6 are specifically designed for the Universal DNA Library Prep Kit. The kit includes four kits for preparing DNA libraries for Illumina platforms. Each kit involves a Universal Adapter as well as 24 unique 8bp indexed DNA Adapters that can be used to differentiate between specimens. All components of the kit have undergone thorough quality check method. At -20°C, the components of the kit can be kept.

Components:

Components of CD DNA Adapters S3-S6 for Illumina

Application:

Special for DNA library preparation for Illumina platforms with CD Universal DNA Library Prep Kit for Illumina (DP001).

Storage:

All the components can be stored at -20℃ for one year.

Specifications:

Application	Used for CD Universal DNA Library Prep Kit for Illumina
Sequencing Platform	Illumina

The structure of libraries prepared with CD DNA Adapters S3-S6 for Illumina are as follows:

5' - Universal Adapter - Insert DNA Sequence - DNA Adapter 96-X - 3'

CD Index Primer for Illumina^®

Both the Universal Adapter and the Index are contained in each DNA Adapter. The sequences of DNA Adapters are as follows:

Name	Sequence
Universal Adapter	5'-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'
DNA Adapter 96-X	5-GATCGGAAGAGCACACGTCTGAACTCCAGTCACIIIIIIIIATCTCGTATGCCGTCTTCTGCTTG-3'

IIIIIIII indicates the index sequences (8 bp). Please input the related Index sequences of DNA Adapters to the Sample Sheet before sequencing as follows:

DSI002-03	Index	DSI002-04	Index	DSI002-05	Index	DSI002-06	Index
DNA Adapter 96-01	AACGTGAT	DNA Adapter 96-25	AGATCGCA	DNA Adapter 96-49	GATAGACA	DNA Adapter 96-73	AATGTTGC
DNA Adapter 96-02	AAACATCG	DNA Adapter 96-26	AGCAGGAA	DNA Adapter 96-50	GCCACATA	DNA Adapter 96-74	ACACGACC
DNA Adapter 96-03	ATGCCTAA	DNA Adapter 96-27	AGTCACTA	DNA Adapter 96-51	GCGAGTAA	DNA Adapter 96-75	ACAGATTC
DNA Adapter 96-04	AGTGGTCA	DNA Adapter 96-28	ATCCT GTA	DNA Adapter 96-52	GCTAACGA	DNA Adapter 96-76	AGATGTAC
DNA Adapter 96-05	ACCACTGT	DNA Adapter 96-29	ATTGAGGA	DNA Adapter 96-53	GCTCGGTA	DNA Adapter 96-77	AGCACCTC
DNA Adapter 96-06	ACATTGGC	DNA Adapter 96-30	CAACCACA	DNA Adapter 96-54	GGAGAACA	DNA Adapter 96-78	AGCCATGC
DNA Adapter 96-07	CAGATCTG	DNA Adapter 96-31	GACTAGTA	DNA Adapter 96-55	GGTGCGAA	DNA Adapter 96-79	AGGCTAAC
DNA Adapter 96-08	CATCAAGT	DNA Adapter 96-32	CAATGGAA	DNA Adapter 96-56	GTACGCAA	DNA Adapter 96-80	ATAGCGAC
DNA Adapter 96-09	CGCTGATC	DNA Adapter 96-33	CACTTCGA	DNA Adapter 96-57	GTCGTAGA	DNA Adapter 96-81	ATCATTC C
DNA Adapter 96-10	ACAAGCTA	DNA Adapter 96-34	CAGCGTTA	DNA Adapter 96-58	GTCTGTCA	DNA Adapter 96-82	ATTGGCTC
DNA Adapter 96-11	CTGTAGCC	DNA Adapter 96-35	CATACCAA	DNA Adapter 96-59	GTGTTCTA	DNA Adapter 96-83	CAAGGAGC
DNA Adapter 96-12	AGTACAAG	DNA Adapter 96-36	CCAGTTCA	DNA Adapter 96-60	TAGGATGA	DNA Adapter 96-84	CACCTTAC
DNA Adapter 96-13	AACAACCA	DNA Adapter 96-37	CCGAAGTA	DNA Adapter 96-61	TATCAGCA	DNA Adapter 96-85	CCATCCTC
DNA Adapter 96-14	AACCGAGA	DNA Adapter 96-38	CCGTGAGA	DNA Adapter 96-62	TCCGTCTA	DNA Adapter 96-86	CCGACAAC
DNA Adapter 96-15	AACGCTTA	DNA Adapter 96-39	CCTCCTGA	DNA Adapter 96-63	TCTTCACA	DNA Adapter 96-87	CCTAATCC
DNA Adapter 96-16	AAGACGGA	DNA Adapter 96-40	CGAACTTA	DNA Adapter 96-64	TGAAGAGA	DNA Adapter 96-88	CCTCTATC
DNA Adapter 96-17	AAGGTACA	DNA Adapter 96-41	CGACTGGA	DNA Adapter 96-65	TGGAACAA	DNA Adapter 96-89	CGACACAC
DNA Adapter 96-18	ACACAGAA	DNA Adapter 96-42	CGCATACA	DNA Adapter 96-66	TGGCTTCA	DNA Adapter 96-90	CGGATTGC
DNA Adapter 96-19	ACAGCAGA	DNA Adapter 96-43	CTCAATGA	DNA Adapter 96-67	TGGTGGTA	DNA Adapter 96-91	CTAAGGTC
DNA Adapter 96-20	ACCTCCAA	DNA Adapter 96-44	CTGAGCCA	DNA Adapter 96-68	TTCACGCA	DNA Adapter 96-92	GAACAGGC
DNA Adapter 96-21	ACGCTCGA	DNA Adapter 96-45	CTGGCATA	DNA Adapter 96-69	AACTCACC	DNA Adapter 96-93	GACAGTGC
DNA Adapter 96-22	ACGTATCA	DNA Adapter 96-46	GAATCTGA	DNA Adapter 96-70	AAGAGATC	DNA Adapter 96-94	GAGTTAGC
DNA Adapter 96-23	ACTATGCA	DNA Adapter 96-47	CAAGACTA	DNA Adapter 96-71	AAGGACAC	DNA Adapter 96-95	GATGAATC
DNA Adapter 96-24	AGAGTCAA	DNA Adapter 96-48	GAGCTGAA	DNA Adapter 96-72	AATCCGTC	DNA Adapter 96-96	GCCAAGAC

Quality Control

16-Hour Incubation: A 50 ul reaction system containing 5 ul of Oligos and 1 μg of Hind III-λ DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis. A 50 μl reaction system containing 5 ul of Oligos and 1 μg of T3 DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis.

Endonuclease Activity: A 50 ul reaction system containing 5 ul of DNA adapters and 1 ug of pX174RF I DNA incubated at 37°C for 4 hours resulted in < 10% conversion to RF Il analyzed by agarose gel electrophoresis.