CD RNA Clean Beads are specially built for RNA purification and are based on SPRI (Solid Phase Reverse Immobilization) technology. It has significantly higher amplicon recovery rates for small and large amplicon sizes >100 bp and is substantially more efficient than traditional filtration DNA/PCR clean-up and RNA/cDNA/RNA purification clean-up. It can produce high-quality double-stranded and single-stranded RNA templates while also removing dNTPs, primers, primer-dimers, and contaminants without the use of salt carryover.
Application
Applicable for RNA purification from in vitro reaction mixtures, i.e., RNA library preparation. Not applicable for direct RNA purification from cells or tissues.
Storage:
All the components can be stored at 2-8℃ for one year.
Specifications:
| Application | Applicable for RNA purification. |
| Sample type | RNA |
- Equilibrate the CD RNA Clean Beads to room temperature and suspend the beads thoroughly by vortexing before use.
- Pipet the CD RNA Clean Beads to the RNA solution. The volumn of beads should be 1.8-fold of the volumn of RNA solution.
- Mix thoroughly by pipetting for 10 times.
- Incubate for 5 min at room temperature for the binding of RNA to beads.
- Place the sample on a magnetic stand for 5 min. Wait until the solution becomes clear. Keep it on the magnetic stand and carefully discard the supernatant without disturbing the beads.
- Keep the sample on magnetic stand, add 200 µl of freshly prepared 80% ethanol to rinse at room temperature and carefully discard the supernatant without disturbing the beads.
- Repeat the step
- Keep the sample on the magnetic stand, open the tube lid and air-dry the beads for 5-10 min.
- Take the sample off from magnetic stand. Add nuclease-free water to elute the RNA. Mix thoroughly by pipetting for 10 times, and then incubate for 5 min at room temperature.
- Place the tube back on the magnetic stand and wait until the solution clarifies (about 5 min). Carefully transfer the supernatant to a new nuclease-free tube without disturbing the beads.
- Store the supernatant at -20℃ or proceed to the next step immediately.
Tips
- Equilibrate the CD RNA Clean Beads to room temperature before use and suspend the beads thoroughly by vortexing every time before pipetting.
- Avoid the contamination of RNase and nucleic acid during the experiment.
- The 80% ethanol should be prepared with nuclease-free water to avoid RNA degradation by RNase.
- When air-drying the beads, do not over-dry it. Over-dried beads with cracks on the surface will lead to reduced elution efficiency of RNA.
- In the protocol Step 10, avoid pipetting beads when transferring the supernatant, i.e. leave behind 2-3 ul of supernatant.
- The CD RNA Clean Beads is compatible for various library prep kits, such as CD mRNA-seq Library Prep Kit for Illumina (RP001), CD Stranded mRNA-Seq Library Prep Kit for Illumina (RP002) or CD Total RNA-seq Library Prep Kit for Illumina (human/mouse/rat) (RP003). Please refer to the protocols of these kits when using the CD RNA Clean Beads for library preparation.
Additional Materials Required
Ethanol (100%)
Nuclease-free water
Magnetic stand
Nuclease-free tubes
