Real-Time-PCR, also called quantitative polymerase chain reaction (qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. Two common methods for the detection of products in Real-Time-PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary template sequence.
RT-PCR (Reverse transcription-PCR) is a PCR process that uses reverse transcriptase to make cDNA from an RNA template. The cDNA then undergoes "normal" PCR processes, under the control of DNA polymerases, resulting in the synthesis of millions of double-stranded DNA molecules representing the sequence of the original RNA.
Commonly, in qRT-PCR, RNA transcripts are quantified by reverse transcribing them into cDNA first, as described above and then qPCR is subsequently carried out. CD One Step qRT-PCR Kits, designed for optimal performance in stringent qRT-PCR reaction conditions, are optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination.
CD Genomics offers a variety of real-time PCR Kits, One Step qRT-PCR Kits and PCR reagents for your research.
We provide high-quality kit products for researchers from across the world, meeting the needs of various nucleic acid-related experiments.
