Full-Length Transcriptome Profiling Combined with Long- and Short-Read Sequencing

CD Genomics provides comprehensive transcriptome profiling services using both short-read (Illumina) and long-read RNA sequencing technologies (PacBio Iso-Seq and ONT nanopore RNA sequencing). Our integrated analysis will facilitate the discoveries of novel transcripts and genes, identification of alternative splicing, and detection of allele-specific expression.

Methodologies for Transcriptome Profiling

Transcriptomics is the study of the transcript catalog of a cell, tissue, or organism under specific conditions (physiological condition, developmental stage, and external environment), involving protein-coding messenger RNA (mRNA) and non-coding RNA (ncRNA), such as transfer RNA (tRNA), ribosomal RNA (rRNA), and among others. Due to the complexity of the transcriptome, transcriptome analysis relies heavily on the availability of high-throughput tools. RNA sequencing using short-read sequencing (Illumina) is currently the standard and widely used approach for transcript profiling. This method is particularly suitable for large-scale, high-throughput studies. While the emerging long-read RNA-seq technologies, such as PacBio SMRT sequencing and nanopore cDNA/RNA sequencing, offer new ways to investigate the transcriptome and its function.

Outstanding Advantages of Our Services

• The short reads correct the long reads to produce more accurate transcriptome information.
• The combination of short-read and long-read RNA-seq enables systematical characterization of the complexity of transcript isoforms.
• The combination of short-read and long-read RNA-seq enables more comprehensive transcriptome analysis.

Workflow of Our Service

Sample Requirements

-Sample type: Blood, tissues, cells, RNA samples (total RNA or poly-A+ RNA), and more.
-RNA amount ≥ 2 μg, RNA concentration ≥ 50 ng/μL, OD260/280=1.8~2.0.
-All RNA samples are validated for purity and quantity.

Sequencing platforms

-Illumina NovaSeq and HiSeq platform.
-PacBio Sequel II sequencing system.
-Nanopore PromethION sequencing platform.

Analysis Pipeline and Contents

Why Do You Choose Us?

• Advanced instruments and highly experienced analysts.
• Proper methods of sample preparation and RNA extraction.
• Customized service to customer satisfaction.
• Complete analytical study reports.
• Our customer service representatives are available 24 hours a day from Monday to Friday.

With multiple specialists and years of experience in RNA-seq, we guarantee you high-quality data and integrated bioinformatics analyses. If you are interested in our short-read and long-read RNA sequencing, please feel free to contact us. We are looking forward to working on your next project.