Whole Genome Bisulfite Sequencing(WGBS)

Inquiry

Whole Genome Bisulfite Sequencing(WGBS) is a next-generation sequencing technology that determines the DNA methylation status of individual cytosines by treating DNA with sodium bisulfite prior to high-throughput DNA sequencing. The DNA methylation status of various genes can reveal information about gene regulation and transcriptional activity. Now, CD Genomics provides you with a variety of WGBS service to meet your research needs.

Specific WGBS Approaches We Offer

Methods

WGBS uses a combination of sodium bisulfite treatment and high-throughput DNA sequencing. Sodium bisulfite protects methylated cytosine or methylcytosine from conversion, while unmethylated cytosine is converted to uracil.
The unmethylated cytosine is further converted to thymine after PCR, which means that the sequencing results show mainly adenine, guanine and thymine bases, with any cytosine bases indicating methylated cytosine sites.

Service content

WGBS	Analysis content
Basic Analysis	Genome alignment Sequencing depth and coverage statistics Quality control of methylation detection Methylation site detection and statistics Sequence feature detection near the methylation site Correlation analysis between samples PCA analysis Differential methylation sites (DMS) analysis Differentially methylated region (DMR) analysis Statistics of DMR identification results DMR structure annotation DMR gene GO enrichment analysis (model species only) DMR gene KEGG enrichment analysis (model species only) Differential Methylation Promoter (DMP) Analysis Differentially methylated promoter (DMP) statistics (model species only) DMP clustering analysis (model species only) DMP Gene GO Enrichment Analysis (Model Species Only) DMP gene KEGG enrichment analysis (model species only)
Senior Analysis	Methylation data SNP detection Tumor purity analysis Identification of methylation transcription factors

Workflow

Whole Genome Bisulfite Sequencing(WGBS)

Service Advantages

Complete platform: one-stop excellent service from sample processing, library building, sequencing to bioinformatics analysis.
High accuracy: mature methylation transformation processing technology precisely achieves methylation status analysis of individual C bases.
Wide coverage: Methylation site detection can be achieved at the whole genome level.
Good reproducibility: stable processing, suitable for comparative analysis among multiple samples.

Our Capabilities

End-to-end package

Includes everything from bisulfite processing to data analysis.

Fast turnaround time

4-8 weeks from sample submission.

Post-completion technical support

Free, high-quality support to assist with data analysis at no additional cost.

Bioinformatics

FASTQ raw data, FASTQC quality control insights, read mapping, methylation calling and differential methylation analysis.

Sample Requirements

We currently accept DNA, cell and tissue samples for our whole genome bisulfite sequencing methyl sequence service. Proper sample preparation as well as appropriate quality control methods will maximize the chances of a successful assay.

Service Process

Our Features

CD Genomics has established a proven epigenomics sequencing platform for a variety of applications. We'll help you design the right WGBS service to aid your scientific research.
CD Genomics is a leading epigenomics sequencing technology company offering a trusted suite of services and solutions, especially with our extensive experience in WGBS service.

Applications

Clinical large sample methylation biomarker screening
Developmental methylation profiling of model organisms
Disease and tumor regulatory mechanisms
Genetic breeding (animal) methylation studies
Methylation of non-referenced genomic species

Deliverables

Related experimental results raw data
Experimental report
Data analysis
Image and result analysis

Why Choose Us?

CD Genomics offers WGBS service to researchers who wish to benefit from this technology. With talented genomics technologists and state-of-the-art facilities, we offer integrated, ready-to-use solutions. We are happy to discuss your project and the potential of applying WGBS service with you. If you would like to know more about this service, please feel free to contact us.

! For research purposes only, not intended for clinical diagnosis or individual assessments.