RIP-Seq
Technology Introduction
RNA immunoprecipitation sequencing (RIP-seq) is a technology for RNA immunohistoscope and combining high-throughput sequencing to capture the RNA of interaction through immune precipitated target protein. The principle is to use antibodies for target protein to precipitate the corresponding RNA-protein complexion, then separate and purify the RNA binding to the complex, and then analyze the target RNA through high-throughput sequencing. The combination of RIP technology and high -throughput sequencing can help researchers understand the dynamic process of regulating networks after transcriptional regulation networks at the whole genome level.
Specific RIP-Seq Approaches We Offer
Analysis Contents
| Comparison statistics |
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| Peak analysis |
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| Peak annotation |
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| Motif analysis |
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Service process
Our Capacity
- One-stop service
Customers only need to provide cells, tissues or RIP RNA, and Cloud Sequence will complete the whole service process from sample processing, library preparation, up-sequencing to data analysis for you.
- Stability
The success or failure of RIP experiments is the key to determine the quality of data. CD Genomics uses a professional technology platform to ensure the success rate and stability of RIP experiments.
- Strict quality control
We incorporate a series of quality control points at each key step of the experiment to monitor the quality of the experiment throughout the process and ensure that our customers get quality data.
- Professional bioinformatics analysis
CD Genomics has a strong bioinformatics team that can meet all kinds of in-depth data analysis requirements of our customers.
Sample Requirements
- Cell, tissue or RNA after IP. other types of samples please ask for details.
- Cell: 5×108
- Tissue: 500 mg
- RNA after IP: >1 μg, OD260/280≥1.8; OD260/230≥1.5 (no obvious degradation, complete and clear characteristic bands of samples detected by electrophoresis, no obvious diffusion or trailing)
- Sample transport: samples were placed in 1.5mL centrifuge f tubes, sealed with sealing film and transported on dry ice.
- Sample preservation: cell samples or fresh tissue blocks were stored at -80°C after freezing in liquid nitrogen.
Service Process
Deliverables
1. Related experimental results raw data
2. Experimental report
3. Data analysis
4. Image and result analysis
5. Bioinformatics analysis results
6. Details in RIP-Seq for your writing
Our Features
- CD Genomics will complete your project on time and efficiently. We have professional after-sales service.
- CD Genomics works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Why Choose Us?
At CD Genomics, our goal is to apply innovative technologies to the analysis of genetic variation and function, making possible research that would have been unthinkable even a few years ago. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. If you would like to know more about this service, please feel free to contact us.