ONT Direct RNA Sequencing
Eukaryotic genes usually produce multiple RNA isoforms (isoforms), which can generate functionally distinct protein variants. Traditional second-generation sequencing is actually a short-read long RNA-seq, which is difficult to obtain complete transcripts and cannot reflect the diversity of isoforms.
Specific ONT Direct RNA Sequencing Approaches We Offer
CD Genomics provides ONT Direct RNA full-length transcriptome sequencing service, based on OxfordNanopore's nanopore sequencing platform, which directly sequences RNA with the advantage of long read length, improves the detection of isoforms, and also directly detects nucleic acid modifications such as m6A and m5C, which has unique advantages in variable shear studies. A single sequencing can meet the needs of several epigenetic transcriptome studies, greatly enhancing the height, depth, accuracy, and efficiency of the study, especially suitable for the study of multi-omics molecular mechanisms and precious clinical samples.
Analysis Content
| Isoform analysis | Expression quantification | Analysis of methylation modifications | Isoform co-quantitative expression analysis |
| 1. Variable shear analysis 2. Fusion gene identification 3. SSR analysis 4. LncRNA analysis 5. PolyA analysis |
1.Transcript quantification 2.Transcript difference analysis 3.Enrichment analysis 4.Protein interaction network |
1.m5C site annotation 2.m6A site annotation 3.Enrichment analysis of methylation modifications 4.m5C differential analysis 5.m6A differential analysis |
1.AltTP analysis 2.Functional diversity analysis ((FDA)) 3.Differential enrichment analysis |
Workflow
Service Advantages
1. As a long-read long sequencing technology, it can reduce the ambiguity caused by re-splicing after the interruption of short-read long sequencing, and the results are more accurate and reliable.
⒉. It can sequence RNA directly to avoid the missing information of RNA modification caused by reverse transcription and PCR amplification.
3. It can directly detect m6A and other base modifications, with optimized algorithm to obtain lossless m6A single-base resolution map.
4. Many data can be obtained in one sequencing: transcriptome profile, m6A modification profile, isoforms, variable shear situation, saving precious clinical samples, saving sample preparation time and effort, and keeping the uniformity of samples.
Sample Requirements
- Species: Human, mouse and rat only, other species to be evaluated
- Total RNA >20 μg
- Cell ＞1 x107
Service Process
Deliverables
- Related experimental results raw data
- Experimental report
- Data analysis
- Image and result analysis
- Bioinformatics analysis results
Applications
1. Full-length sequencing of individual RNA molecules at the single-molecule level..
2. Identify longer transcripts and study transcript isoforms
3.Mapping m6A modifications on the transcriptome with resolution up to unit point.
4. ONT direct RNA sequencing can detect RNA variable shear.
Our Features
- CD Genomics has established a proven epigenomics sequencing platform for a variety of applications. We'll help you design the right oxBS-seq service to aid your scientific research.
- CD Genomics is a leading epigenomics sequencing technology company offering a trusted suite of services and solutions, especially with our extensive experience in oxBS-seq service.
Why Choose Us?
CD Genomics is a professional ONT Direct RNA Sequencing service provider supporting a wide range of sequencing services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of epigenomics sequencing service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.