MRE-Seq Service

MRE-Seq is a protocol to study DNA methylation using methylation-sensitive restriction enzymes (MSREs) on genomic DNA. mRE-seq enriches unmethylated DNA and can cover 1.7 million CpG sites in the human genome. In this approach, gDNA is first digested separately with different MSREs. The library is prepared and sequenced based on size-selected restriction fragments. Deep sequencing allows for accurate detection of methylation sites in the genome. Now, CD Genomics provides you with a variety of MRE-Seq service to meet your research needs.  

Specific MRE-Seq Service Approaches We Offer

• In the MRE-Seq workflow, MRE cleaves unmethylated CpG sites in genomic DNA, and the resulting DNA fragments are size-selected and sequenced. During the MRE-Seq process, we strictly control each step to ensure reliable experimental data are obtained.
• CD Genomics provides not only stand-alone MRE-Seq services, but also identifies different methylation regions (DMRs) data by jointly modeling MRE-Seq and MeDIP-Seq.

Services Advantages

• Both MRE-Seq and MeDIP-Seq are technologies that measure DNA methylation at the genome-wide level.
• MRE-Seq and MeDIP-Seq represent complementary ways of enriching the unmethylated portion of the genome or the methylated portion of the genome.
• MRE digestion combined with sequencing to predict genome-wide DNA methylation levels and identify unmethylated CpG sites at single CpG site resolution.

Our Capabilities

• Customers only need to provide samples, and we complete the whole process service from MRE digestion, library preparation, up-sequencing to data analysis.
• We have strict quality control for each step of MRE-Seq to ensure customers get high quality data, guaranteeing more than 80% of base quality scores ≥Q30, exceeding Illumina's official guarantee of ≥75%.

Workflow

Sample Requirements

We currently accept DNA, cell, and tissue samples for our MRE-Seq service. Sample sources include human, animal, plant, and microbial.

Service Process

Deliverables

1. Related experimental results raw data

2. Experimental report

3. Data analysis

4. Image and result analysis

5. Bioinformatics analysis results

6. Details in MRE-Seq Service for your writing

Our Features

Why Choose Us?

CD Genomics is a company that provides professional and comprehensive MRE-Seq services. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.