MeRIP-Seq
Technology Introduction
m6A methylation is an important epigenetic modification in post-transcriptional regulation, and it is commonly found in viruses and eukaryotes. m6A methylation by MeRIP-seq (high-throughput sequencing) is a genome-wide detection method for m6A modifications, which has the advantages of being able to specify the level of modification of each gene and to compare groups with each other. Next, let me bring you a detailed understanding of m6A and MeRIP-seq.
Specific MeRIP-Seq Approaches We Offer
CD Genomics provides MERIP technology suitable for different scientific research needs:
- m6A methylation-constant mRNA methylation sequencing (MeRIP-seq)
- m6A methylation-constant mRNA +LNCRNA methylation sequencing (LNC-MeRIP-seq)
- m6A methylation-trace MRNA +LNCRNA methylation sequencing (Micro-LNC- MeRIP -seq)
- High-throughput m6A methylation-constant mRNA methylation sequencing (MeRIP -seq2)
Analysis Contents
| MeRIP-seq analysis content | |
| Standard analysis | Quality control and assessment: 1. Data quality control 2. Comparison statistics 3. Insert fragment length statistics 4. Statistics of whole genome coverage 5. Sample saturation curve |
| Statistics of M6A rich area (Peak): 1. Appraisal and annotations of the m6A enrichment area (Peak) 2. Basic statistics in the m6A enrichment area 3. The distribution of m6A enrichment area on chromosome 4. The distribution of the m6A enrichment area on gene components 5. Functional enrichment analysis of m6A enrichment area association genes 6. The distribution density of the m6A enrichment area on gene components 7. MOTIF identification in the m6A modification area |
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| Differential m6A modification identification and statistics: 1. Analysis of the overlapping situation of samples in the group 2. Analysis of the overlapping situation of the sample peak association gene in the group 3. Differential m6A modification identification and annotation 4. Basic statistics of different m6A modification 5. Differential m6A modification distribution on chromosomes 6. Differential m6A modification of distribution density on gene components 7. Differential MOTIF identification in the m6A modification area |
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Workflow
Service Advantages
- Low starting volume: the sample starting volume can be reduced to 10-20 μg, with a minimum of 1 μg of total RNA.
- Transcriptome wide: mRNA and lncRNA can be detected simultaneously.
- Sample requirements: can be used for m6A detection in animals, plants, cells and tissues
- High reproducibility: IP enrichment is highly reproducible, maximizing the reduction of antibody enrichment bias
- Wide range of applications: widely used in research fields such as tissue development, stem cell self-renewal and differentiation, heat shock or DNA damage response, cancer occurrence and development, drug response, etc.
Sample Requirements
- Sample type: de -protein and the complete total RNA sample after DNASE processing
- Total sample: ≥10 μg
- Sample concentration: Suggestion ≥250 ng/ μl
- Sample integrity: RIN ≥ 7, Ratio 28S/18S ≥ 0.7, certain sources of samples (such as body liquid samples, insect samples, aquatic biological samples, etc.)
Service Process
Deliverables
1. Related experimental results raw data
2. Experimental report
3. Data analysis
4. Image and result analysis
5. Bioinformatics analysis results
6. Details in MERIP-Seq for your writing
Our Features
- We have accumulated many years of essence in the field of MeRIP-Seq service, helping customers to speed up project development and improve the overall success rate of the project.
- At CD Genomics, simply let us know your specific project needs. We will suggest the best strategy for you.
Why Choose Us?
CD Genomics is a company that provides professional and comprehensive MeRIP-Seq services. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.