DAP-Seq
Technology Introduction
DAP-SEQ is a technology based on DNA affinity purification and identification of TFBS through in vitro expression of transcription factors. It has the advantage of high throughput and is not limited by antibodies and species. Since its advent, DAP-SEQ has been widely used in transcriptional regulation and epigenomics research.
Principle: The protein and DNA expressed in vitro are affinity purified, and the DNA bound to the protein is eluted for high-throughput sequencing.
Specific DAP-Seq Approaches We Offer
CD Genomics' experienced team of experts perform quality control at every step of the process to ensure comprehensive and accurate results. Our DAP-Seq workflow is outlined below and includes library preparation, protein expression, DNA affinity purification, sequencing and bioinformatics analysis.
Service process
We offer custom bioinformatics analysis, including but not limited to:
- Data quality control
- Comparison with reference genomes using mapping statistics
- Peak calling and annotation
- Differential peak annotation
- Functional analysis of peak-associated genes
- Gene sequence prediction
- Pathway analysis
- Visualization of results
Technical process: construct the CDS sequence encoding transcription factors into the vector containing affinity tags, construct the protein expression vector, carry out protein expression in vitro, and form the fusion protein of transcription factors and affinity tags; The genomic DNA of the sample was extracted, a DNA library was constructed, and then the transcription factors with affinity tags expressed in vitro were combined with the DNA library, and then the combined DNA was eluted and sequenced on the computer.
Services Advantages
- Suitable for high-throughput sample processing of the entire TF ORF clone collection.
- Does not require specific antibodies against each transcription factor, relatively cost-effective for all eukaryotes
- DAP-Seq preserves many of the tissue/cell lineage-specific secondary modifications and features present genome-wide.
Sample Requirements
DNA samples: 5 µg genomic DNA and 1 µg Halo-ORF plasmid DNA.
Applications
- Transcription factor binding sites and functional studies.
- Identification of histone modification types at specific DNA strand locations.
- Study of histone modifications in relation to gene expression.
Service Process
Deliverables
1. Related experimental results raw data
2. Experimental report
3. Data analysis
4. Image and result analysis
5. Bioinformatics analysis results
6. Details in DAP-Seq for your writing
Our Features
- CD Genomics has a group of technical experts specializing in DAP-seq services. Our extensive research and technical experience can help our customers solve their DAP-seq technical problems.
- CD Genomics has a proven, advanced DAP-seq technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.
Why Choose Us?
CD Genomics is a professional DAP-seq technology provider supporting a wide range of DAP-seq services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of DAP-seq service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.