Custom NGS Panel

CD-Genomics is a company with rich experience in targeted gene panel sequencing which is used to accurately detect gene variations related to diseases.

CD-Genomics provides tests for mutations in at least 68 diseases and thousands of genes. Our scientists summarize disease-related genes based on the literature related to diseases. According to the genes that the customer is interested in, we use the targeted NGS sequencing method to analyze SNP, CNV and InDels. There are two targeted sequencing methods available: amplicon-based approaches and hybridization capture-based approaches.

• Amplicon sequencing: Specific primers are designed for PCR amplification for the target region of interest, multiplex up to 20,000+ amplicons in each reaction. Sequencing adapters are ligated or added by a second PCR amplification and the targeted DNA library is constructed. High-throughput sequencing is then performed.
• Hybridization capture-based target enrichment: At present, the main method is hybrid capture sequencing based on liquid phase. Based on the principle of base complementary pairing, nucleic acid probes are designed for specific regions of the genome of interest. The target region hybridization enrichment of DNA/RNA library is carried out based on liquid phase environment, and the specific genetic mutation or somatic mutation in the target region are detected by high-throughput sequencing technology.