Sample Submission GuidelinesThe NextSeq Sequencing Systems from Illumina is a powerful and versatile system for high-throughput sequencing applications. It’s designed to deliver rapid and accurate results across various sequencing workflows, making it a popular choice for research, clinical, and applied genomics projects. This series is available in a variety of models Illumina NextSeq500, NextSeq550, NextSeq1000 and NextSeq 2000 sequencing systems.
At CD Genomics, we utilize Illumina, MGI, PacBio SMRT and Oxford Nanopore sequencing platforms for our sequencing services. CD Genomics also offers its expertise in SNP and microsatellite genotyping on a wide range of platforms.
Please refer to our Sequencing Platform for more information.
NextSeq 500 vs. NextSeq 1000
Table 1 NextSeq 500 vs. NextSeq 1000
| Systems | NextSeq 1000 | NextSeq 550 |
| Chip Scanning Capability | None | – CytoSNP-850K Beadchip – CytoSNP-12 BeadChip – HumanKaryomap-12 BeadChip |
| Light Source | Laser wavelengths of 449 nm, 523 nm, 820 nm | LED wavelengths of 520 nm, 650 nm |
| Instrument Dimensions | 60 cm (Width) x 65 cm (Depth) x 60 cm (Height) Weight: 141 kg | 53.3 cm (Width) x 63.5 cm (Depth) x 58.4 cm (Height) Weight: 83 kg |
| Operating Modes | – 2x50bp/40Gb – 2x100bp/80Gb – 2x150bp/120Gb |
High-throughput flow cell: – 1x75bp/30Gb – 2x75bp/60Gb – 2x150bp/120Gb Mid-throughput flow cell: – 2x75bp/19Gb – 2x150bp/39Gb |
| Data Output & Quality | – 40Gb, Q30>85%, 13h – 120Gb, Q30>75%, 29h |
– 16Gb, Q30>80%, 15h – 120Gb, Q30>75%, 29h |
How to Choose Between NextSeq 1000 and NextSeq 550?
If you need lower startup throughput, chip scanning capability, or have in vitro diagnostic (IVD) requirements, then choose NextSeq 550. If you prefer lower sequencing costs, faster analysis speed, or intend to conduct single-cell sequencing analysis, then choose NextSeq 1000.
NextSeq 2000 Sequencing System
The NextSeq 2000 is exceptionally well-equipped to address future demands, offering robust sequencing capabilities for high-throughput applications.
Table 2 NextSeq 2000 Sequencing System
| Feature | Description |
| System Type | All-in-one benchtop sequencer |
| Sequencing Reagents | 10 different sequencing reagents available |
| Maximum Throughput | 360 Gb in a single run |
| Maximum Read Length | 600 cycles |
| Applications Supported | – 16S metagenome sequencing – Exome sequencing – Disease analysis |
| Fast Genome Analysis Server | On-board server for accurate and fast genome analysis |
| Data Compression | Lossless compression reduces data storage space by up to 80% |
| Integrated Reagent Cartridges | Simplifies operation with integrated reagent cartridges |
| Modular Design | Features a no-clean dry system for fast and environmentally friendly operation |
Applications of NextSeq 2000
- Single-cell Gene Expression Analysis
Uncover the transcriptome of individual cells, providing a nuanced understanding of cell-to-cell variability. Explore diverse biological properties within complex tissues and decipher how different cell subpopulations respond to environmental cues. With P2 reagent, analyze up to 10 samples in approximately 13 hours, or utilize P3 reagent to process 25 samples in about 19 hours.
- Whole Exome Sequencing
Offering a cost-effective alternative to whole-genome sequencing, exome sequencing efficiently identifies coding variants essential for various applications such as population genetics, genetic diseases, and cancer research. With P2 reagent, analyze 16 samples in approximately 21 hours, or utilize P3 reagent to obtain 40 complete exomes in about 33 hours.
Comprehensive detection of genes across all organisms within complex samples. Assess bacterial diversity, determine microbial abundance in diverse environments, or study unculturable microorganisms beyond the reach of conventional methods. Process data from 8 samples in approximately 29 hours with P2 reagent, or scale up analysis with P3 reagent to handle data from up to 20 samples in about 48 hours.
Related Services
Whole Genome Sequencing
Whole Exome Sequencing
Metagenomic Shotgun Sequencing