Blog Archives

Introduction to Mitochondrial DNA (mtDNA) Mitochondrial DNA (mtDNA) is a special type of genetic material found in mitochondria, the energy-producing parts of cells. These cellular organelles function as primary energy-generation mechanisms, predominantly responsible for adenosine triphosphate (ATP) production—the fundamental energy mechanism supporting biological processes. Distinguished from nuclear genomic material, mtDNA exhibits a unique genetic configuration […]

Originating from Latin, the expression "de novo" signifies initiating processes without dependence on preexisting information or references. Within genomic research, de novo sequencing represents a transformative methodology that empowers scientific investigators to decode entire genetic blueprints independently of reference frameworks. This groundbreaking approach has fundamentally reshaped genetic exploration, enabling unprecedented discoveries of molecular variations and […]

In the realm of genomics, the length of sequencing reads is a pivotal factor influencing the quality and depth of genetic data. Whether engaged in whole-genome sequencing, RNA sequencing (RNA-seq), or the detection of structural variants, comprehending the impact of read length on research outcomes is imperative. This treatise elucidates the importance of sequencing read […]

In the realm of modern biology, unlocking the complexities of genomes has been significantly propelled by next-generation sequencing (NGS) technologies. Among these, the sequencing-by-synthesis (SBS) chemistry, in tandem with the esteemed DRAGEN Bio-IT platform, stands out for its unparalleled accuracy in delivering whole genome sequencing (WGS) data. However, the quest for understanding the entirety of […]

The NextSeq Sequencing Systems from Illumina is a powerful and versatile system for high-throughput sequencing applications. It’s designed to deliver rapid and accurate results across various sequencing workflows, making it a popular choice for research, clinical, and applied genomics projects. This series is available in a variety of models Illumina NextSeq500, NextSeq550, NextSeq1000 and NextSeq […]

Pyrophosphate sequencing represents an innovative enzymatic cascade chemiluminescence sequencing method driven by four key enzymes: DNA polymerase, ATP sulfurylase, luciferase, and adenosine triphosphate bisphosphatase (apyrase). This cutting-edge technology enables real-time detection of biological light signals emitted during DNA synthesis, setting a groundbreaking precedent for simultaneous sequencing and synthesis. In the realm of early tumor screening […]

What is Exon? A coding sequence within a fragmented gene, an exon (expressed region) constitutes a vital segment of a eukaryotic gene. It remains conserved post-splicing and serves as the basis for protein expression during protein biosynthesis. These nucleotide sequences are present in both the initial transcription product and the mature RNA molecule. Genome encompasses […]

The study of population evolution relies on advanced genomic techniques like whole-genome resequencing or other sequencing methods. These technologies enable the acquisition of genomic data from diverse subpopulations within a species. By extracting extensive variant information such as single nucleotide polymorphisms (SNP), insertions/deletions (InDel), structural variations (SV), and copy number variations (CNV), researchers delve into […]

What is Agrobacterium-Mediated Plant Transformation? Agrobacterium, a soil-dwelling bacterium, can chemotactically infect a wide array of dicotyledonous plants. Exploiting wounded areas of the plant, Agrobacterium induces the formation of crown gall tumors or hairy roots. Within Agrobacterium‘s Ti and Ri plasmids resides a crucial fragment of transfer DNA known as T-DNA. This T-DNA has the remarkable capability […]