What is Pyrophosphate Sequencing (Pyrosequencing)?
Pyrophosphate sequencing represents an innovative enzymatic cascade chemiluminescence sequencing method driven by four key enzymes: DNA polymerase, ATP sulfurylase, luciferase, and adenosine triphosphate bisphosphatase (apyrase). This cutting-edge technology enables real-time detection of biological light signals emitted during DNA synthesis, setting a groundbreaking precedent for simultaneous sequencing and synthesis. In the realm of early tumor screening […]