Yearly Archives: 2024

What is Lentiviral Transduction Lentiviral transduction is a method employed to introduce a target gene into recipient cells via lentiviral vectors, facilitating its stable expression. When compared to other viral vectors, lentiviral vectors offer distinct advantages, particularly in integrating genes into non-dividing cells. Typically derived from a modified version of the human immunodeficiency virus (HIV), […]

Introduction: What is KEGG and Why is it Important for Researchers The Kyoto Encyclopedia of Genes and Genomes (KEGG) is an advanced computational tool in molecular biology research. Originated by Kanehisa Laboratories during 1995, this innovative database emerged as a comprehensive genomic repository, dedicated to advancing deep understanding of complex biological systems. Through continuous evolution, […]

Introduction to Mitochondrial DNA (mtDNA) Mitochondrial DNA (mtDNA) is a special type of genetic material found in mitochondria, the energy-producing parts of cells. These cellular organelles function as primary energy-generation mechanisms, predominantly responsible for adenosine triphosphate (ATP) production—the fundamental energy mechanism supporting biological processes. Distinguished from nuclear genomic material, mtDNA exhibits a unique genetic configuration […]

Originating from Latin, the expression "de novo" signifies initiating processes without dependence on preexisting information or references. Within genomic research, de novo sequencing represents a transformative methodology that empowers scientific investigators to decode entire genetic blueprints independently of reference frameworks. This groundbreaking approach has fundamentally reshaped genetic exploration, enabling unprecedented discoveries of molecular variations and […]

In the realm of genomics, the length of sequencing reads is a pivotal factor influencing the quality and depth of genetic data. Whether engaged in whole-genome sequencing, RNA sequencing (RNA-seq), or the detection of structural variants, comprehending the impact of read length on research outcomes is imperative. This treatise elucidates the importance of sequencing read […]