The occurrence, growth and metastasis of tumors are closely related to the internal and external environment in which they are located. The tumor microenvironment (TME) includes not only the structure, function and metabolism of the tissues in which tumor cells are located, but is also related to the intrinsic environment of tumor cells themselves. The tumor immune microenvironment (TIME) is a key determinant of tumor-immune interactions. Analysis of the composition of the tumor immune microenvironment and its development in tumorigenesis is relevant to tumor research.
Figure 1. Critical insights at specific stages of the cancer immunity cycle
The use of NGS-based genomic approaches to analyze the tumor immune microenvironment has important implications for the study of immuno-oncology mechanisms. At CD Genomics, assessing the expression profile of the tumor microenvironment by NGS allows for better assessment of antitumor activity and efficacy of responses.
Here, CD Genomics provides you with NGS-based tumor immune microenvironment analysis services that enable quantification of the relative infiltration of innate and adaptive immune cells (CD8+, CD4+, T cells, B cells, Treg cells, NK cells, macrophages, monocytes, neutrophils) by proprietary immune cell type-specific gene expression profiles. We achieve monitoring of tumor progression, drug efficacy and resistance by analyzing and assessing the tumor immune microenvironment. In addition to this, NGS-based sequencing services include identifying biomarkers for patient selection, predicting the likelihood of response to immune checkpoint inhibitors and monitoring the persistence of response.
01. Sequencing sample requirements
| Sample Type | DNA Amount | DNA Concentration | Purity |
|---|---|---|---|
| Total DNA from cells, blood, saliva, tissue, formalin-fixed samples, fresh frozen tissue samples, paraffin-embedded tissues, cancer liquid biopsies, etc. | ≥ 2 μg | ≥ 50 ng/μL | OD260/280=1.8 - 2.0; no degradation, no contamination |
02. Library construction and NGS
Highly effective and cost-saving NGS-based Illumina / MGI platform
03. Bioinformatics Analysis
We offer a robust NGS-based one-stop shop for sample-to-result analysis of the tumor microenvironment compatible with different sample quantities and quality. The following are a series of applications of tumor microenvironment analysis from CD Genomics that can greatly advance the mechanistic study of tumor immunology.
CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' tumor microenvironment profiling services in the field of immuno-oncology, please feel free to contact us now.
CD Genomics is transforming biomedical potential into precision insights through seamless sequencing and advanced bioinformatics.
