Overview of whole genome sequencing

The transcriptome is the ensemble of all transcription products, including mRNA, rRNA, tRNA and other non-coding RNAs, in a cell under specific physiological conditions and time. The abundance and proportion of these RNAs when subjected to internal and external influences can reflect the current physiological metabolic state of the cell. The purpose of transcriptome sequencing is to determine the relative abundance of various types of RNA molecules and thus indirectly analyze the response of the organism during different internal and external environmental changes. CD Genomics has high precision and low cost NGS capability in transcriptome quantification and monitoring, and its potential in the biopharmaceutical field is unlimited and yet to be explored, such as deeper study of drug mechanism of action, resistance mechanism of the organism and rational drug combination.

The workflow of transcriptome sequencing service

Here, CD Genomics offers you NGS-based transcriptome analysis services ranging from analysis of differential gene expression to detection of variable splicing and unknown transcripts. Our transcriptome sequencing analysis services can help you maximize your potential in the biopharmaceutical field by actively promoting the use of transcriptome sequencing in disease mechanism research and drug development.

01. Sequencing sample requirements

02. RNA Library construction and NGS

NGS-based Illumina / MGI platform

03. Bioinformatics Analysis

• De novo assembly and reference genome mapping
• Gene ontology annotation
• Expression profile identification and quantification
• Sequencing depth and coverage assessment
• Detection of differentially expressed genes
• Alignment of trimmed sequencing data
• RNA variant prediction

Advantages of our transcriptome sequencing service

• High-resolution sequencing technology allows precise quantification and detection of RNA, quantifying virtually all transcripts in the cell
• Highly sensitive sequencing technology allows for the discovery of new or rare transcripts
• Fast-track option available on request for cycle time
• Low cost but efficient and high-quality sequencing analysis services
• Low RNA content requirements but high sequencing depth and high sequencing coverage

Applications in pharma research

CD Genomics offers high-quality transcriptome sequencing analysis, primarily for biopharmaceutical applications, that can help drug developers maximize the potential of their pipelines. Detailed analysis of differential RNA expression and identification of biomarkers across a broad transcriptional range can provide novel mechanisms at the molecular level for the generation and progression of various diseases. At the same time, research into new drug development and new approaches can be further advanced.

CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' transcriptome sequencing service in the field of pharma research, please feel free to contact us now.