Target region sequencing focuses on the gene sequences of interest to the client, and generally these specific regions are associated with disease occurrence. Compared to whole genome sequencing and whole exome sequencing, target region sequencing has a deeper sequencing depth and more comprehensive coverage for a given region. Therefore, target region sequencing is more efficient and cost effective when sequencing a given region or capturing a specific region is required. The type of sequencing service needs to be selected on an as-needed basis, and CD Genomics has been committed to providing NGS-based target region sequencing services to assist you in the development of biomarkers and drug development studies. Our highly sensitive target sequencing service provides more comprehensive, detailed and reliable data analysis results while maintaining high efficiency.
CD Genomics' target region sequencing has been designed to help you obtain region-specific information on point mutations, insertions or deletions (InDels), copy number variations (CNVs), and gene rearrangements for biomarker discovery studies. The process of target region sequencing is shown in the figure below, which mainly includes target region capture panel customization, sample preparation, library building, up-sequencing and bioinformatics analysis. CD Genomics offers custom target region capture panels as well as a range of standardized target region panels to choose from.
01. Sequencing sample requirements
| Sample Type | DNA Amount | DNA Concentration | Purity |
|---|---|---|---|
| Total DNA from all types of cells, blood, saliva, tissue, formalin-fixed samples, fresh frozen tissue samples, paraffin-embedded tissue, cancer liquid biopsy samples, colonies, swabs, etc. | ≥ 200 ng | ≥20 ng/μL | OD260/280=1.8 - 2.0; no degradation, no contamination |
02. Library construction and NGS
The important application of CD Genomics' target region capture sequencing service is the development of biomarkers for various diseases, such as genetic testing for single gene diseases, cancer susceptibility gene testing, and medication guidance gene testing.
CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' targeted region sequencing services in the biomarker discovery research, please feel free to contact us now.
CD Genomics is transforming biomedical potential into precision insights through seamless sequencing and advanced bioinformatics.
