Overview of single cell genome sequencing

With the microcosm of life science research, cell population-based research methods are no longer suitable for certain research areas (e.g., tumor heterogeneity, early embryonic development, etc.). By sequencing at the single cell level, single-cell genome sequencing solves the problem of not being able to obtain information on heterogeneity among different cells with tissue samples or having too few samples for conventional sequencing, and provides a new direction for scientists to study the behavior and mechanism of individual cells. At CD Genomics, we are committed to providing high-quality single-cell sequencing services to support pipeline research for pharmaceutical and life science companies. The principle of single cell whole genome sequencing technology is to amplify trace amounts of whole genomic DNA from isolated individual cells to obtain high coverage of the complete genome followed by high throughput sequencing for revealing cell population differences and cellular evolutionary relationships. CD Genomics' scalable sequencing service process maintains strict QC standards to ensure accurate and reliable data for you.

Figure 1. Schematic representation of homogeneity and heterogeneity between samples

The workflow of single cell genome sequencing service

CD Genomics offers NGS-based single-cell sequencing analysis to study the heterogeneity of genetic information. The process of single cell sequencing is shown in the figure below, which mainly includes cell isolation, library preparation, NGS and bioinformatic blood analysis. The entire sequencing process is performed under strict QC standards and with the participation of experienced experts.

01. Sequencing sample requirements

02. Single cell library construction and NGS

NGS-based Illumina / MGI platform

03. Bioinformatics Analysis

• Sequencing depth and coverage statistics
• Variant calling SNP / InDel /SV / CNV analysis
• Visual annotation of intercellular heterogeneity
• Population genetics analysis

Advantages of our single cell genome sequencing service

• Optimized workflow to maximize single-cell sequencing project flexibility, speed and data accuracy
• High-throughput NGS-based technology platform to ensure short cycle times but high quality
• High sample inclusivity and platform flexibility allows for delivery of all sample types
• Efficient and convenient one-stop sequencing service, all you need to do is provide the sample to be sequenced and we will provide you with a completed and detailed analysis report.

Applications in pharma research

The key advantage of CD Genomics' single cell sequencing service is that the analytical assay yields heterogeneous information that is not available from sequencing of mixed samples. This heterogeneity information is focused on disease mechanisms and drug discovery, and is applied to the analysis of sample heterogeneity in complex diseases, the study of complex disease mechanisms and development, the discovery of new biomarkers, and the evolutionary analysis of diseased cells during disease development.

CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' single cell genome sequencing service in the field of pharma research, please feel free to contact us now.