Overview of metagenomics sequencing

Metagenomics is the study of genetic material recovered directly from environmental samples, such as soil or water samples. Metagenome sequencing allows researchers to comprehensively sample all genes in all organisms present in a given complex sample. Metagenome sequencing not only provides information on species composition and abundance, but also on functional genes, genetic differences between samples, and metabolic pathways, providing a rich data resource for mining bioactive products such as novel enzymes and other biocatalysts. CD Genomics offers the following two types of macrogenomic sequencing methods.

• Shotgun metagenomic sequencing is an integrated method of looking at all genes of all microorganisms in a sample, similar to whole genome sequencing. This method allows you to assess microbial diversity and detect the abundance of species under different conditions. In shotgun sequencing, the total DNA of a sample is isolated and randomly sheared into smaller fragments before sequencing.
• Amplicon metagenomics sequencing is similar to targeted sequencing and can effectively identify microbial diversity in a sample by analyzing operational taxonomic units (OTUs). It works by PCR amplification of conserved genes such as 16S, 18S, ITS and CO1 and sequencing of the resulting amplicons.

The workflow of metagenomics sequencing service

CD Genomics offers NGS-based metagenomics sequencing services to help researchers explore the unique biology of individual cells in complex tissues and understand the responses of cell populations and cell subpopulations to various time-dependent processes of changes in the physiological environment, such as differentiation, proliferation, and disease progression.

01. Sequencing sample requirements

02. Library construction and NGS

NGS-based Illumina / MGI platform

03. Bioinformatics Analysis

• Gene function annotation
• Comparative analysis between multiple samples
• Amplicon OTU analysis
• Reading classification

Advantages of our metagenomics sequencing service

• Sequencing targets a wide range of microorganisms, all in a single run. There is no need for you to specifically isolate and culture microorganisms or amplify target regions of prokaryotes or eukaryotes.
• CD Genomics has extensive experience in providing metagenome sequencing services to help clients obtain information about many different sample types.
• Low cost but high quality NGS-based macro-sequencing analysis master helps you to get comprehensive data analysis results ultimately.

Applications in pharma research

In general, metagenome sequencing has application advantages in microbial community identification. In our accredited state-of-the-art laboratories, CD Genomics offers you NGS-based metagenomics sequencing services, including complete workflow solutions, from DNA isolation and sequencing to advanced bioinformatics analysis. The main applications of metagenomics sequencing in the pharmaceutical field are gut microbiome analysis, infectious disease mechanism research and monitoring, disease target discovery, and improving the drug discovery and development process.

CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' metagenomics sequencing service in the field of pharma research, please feel free to contact us now.