Lentiviruses are a class of retroviruses that integrate into the chromosomes of host cells for long-term stable expression and, in contrast to other retroviruses (e.g., adenoviruses), can infect not only dividing but also non-dividing cells. Based on these advantages, lentiviral vectors have been widely used as exogenous gene transfer vectors. However, it is not yet possible to use lentiviral vectors to insert target genes into specific loci, and therefore there is a risk of insertional mutations causing cancer. Adeno-associated virus (AAV) is the simplest class of single-stranded DNA-deficient viruses with good safety profile, wide host cell range and long expression time in vivo. One of the possible risks of virus vector-mediated random insertion of exogenous genes is that they may integrate into proto-oncogenes or oncogenes of host cells and cause insertional mutagenesis, leading to activation of oncogenes or suppression of oncogenes, thereby inducing carcinogenesis. These potential side effects are of great concern, and there is an urgent need to develop universal integration site assays to assess the safety of viral vector.
CD Genomics can provide you with NGS-based lentiviral integration site analysis services, enabling specific capture of integration site sequences and comprehensive bioinformatics analysis. The entire process of lentiviral integration site analysis follows strict QC standards to ensure the highest quality data and reliable data analysis reports for you.
CD Genomics' lentiviral integration site analysis services are centered around the highly efficient Illumina sequencing technology platform and include sample preparation, library construction, second-generation sequencing and comprehensive bioinformatics analysis. The entire sequencing process is maintained under strict QC standards with the involvement of our experienced team of experts.
01. Sequencing sample requirements
| Sample Type | DNA Amount | DNA Concentration | Purity |
|---|---|---|---|
| Any retroviral infected cells from, blood, saliva, tissue, formalin-fixed samples, fresh frozen tissue samples, paraffin-embedded tissues, cancer liquid biopsies, etc. | ≥ 200 ng | ≥ 20 ng/μL | OD260/280=1.8 - 2.0; no degradation, no contamination |
02. Library construction and NGS
NGS-based Illumina / MGI platform
03. Bioinformatics Analysis
Lentiviral integration site analysis is a very important part of viral vector development, which is essential for gene integration validation, integration mechanism study, safety assessment and efficacy prediction. CD Genomics provides you with high-efficiency NGS-based integration site analysis, mainly for lentiviral vector design and development.
CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' lentiviral integration sites analysis service, please feel free to contact us now.
CD Genomics is transforming biomedical potential into precision insights through seamless sequencing and advanced bioinformatics.
